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- Part II. Transcription as a Process 4. Use the below "transcription bubble model" of a gene during the process of transcription to do following: • Label the 3' and 5' ends of all polynucleotides appropriately • Label the mRNA transcript • Label the template strand HillT لليليلا UFUP 5. What protein is responsible for making the "bubble" in the DNA shown above?Show the structure of a DNA where the lead strand is ATCG. Show H-, glycosidic, phosphoester bondings.4. Differentiate replication, transcription and translation by describing the changes which occur in each process.ACTIVITY 2: Protein Synthesis 1 2. 4. DNA: 5. 3' A G C C GTA GA ATT 3. Carefully indicate the codons present in the mRNA strand from question 2. 5' Using this strand of DNA as a template, DRAW A PICTURE of the complete DNA molecule. Include ALL parts of the DNA molecule. You do not need to draw your molecule with atomic accuracy. Now draw a complete picture of the mRNA strand that will be made from this DNA. Label the 5' and 3' ends of your mRNA strand. (Use the given DNA strand at the top of this page as your template...) Draw a COMPLETE picture of all the tRNA molecules that will match up with the codons from the previous question. Include ALL APPROPRIATE amino acids in your picture, and do not mix up their order! Draw a picture of the completed protein coded for by this strand of DNA (abbreviations are fine). Show the amino acids in the same order they would be observed in the finished protein.
- Group B 1. Why Phosphate bond is important in DNA or RNA structure? 2. Why G-C forms three hydrogen bonds while A-T forms only two? 3. Briefly explain the importance of poly A tail of mRNA. 4. What is nonsense mutation? Describe with an example. 5. Write the importance of polypeptide bond. 6. Is this possible to create a DNA sequence from an RNA and how? 7. Which gene mutation is much severe and why? 8. What are the possible outcome of Chromosome mutation? 9. Define the domain and motif of protein. Why they are required?21. The difference between a nucleoside and nucleotide is: Group of answer choices 1. A nucleoside consists of the sugar with a nitrogenous base, whereas a nucleotide has a sugar with a nitrogenous base and phosphate groups attached to the sugar. 2. Nucleotides contain deoxyribose sugar and nucleosides contain ribose sugar 3. Nucleotides are involved in eukaryotic DNA replication, while nucleosides are used in bacterial DNA replication 4. A nucleotide consists of the sugar with a nitrogenous base, whereas a nucleoside has a sugar with a nitrogenous base and phosphate groups attached to the sugar.II. Use the eukaryotic gene DNA sequence below to answer the following questions: 1 11 CGACTTACTG 51 TGGACGCGCC 101 Genetic Code: First letter U с c. Where is the 3' UTR? Circle one. 70-111 14-60 C Polyadenylation signal in the corresponding mRNA: Kozak's sequence in the corresponding mRNA: Start (initiation) codon in the corresponding mRNA: Stop (termination) codon in the corresponding mRNA: UUU UUC UUA UUG] CUU CUC CUA CUG 61 AUU AUC AUA AUG لالا GUU GUC GUA GUG GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA 111 GTCATTCAGC GTAGTCTGAT GCCAGTCGAC TGCATTGGAC ACCGGTTACA a. Write the corresponding sequences, circle & label them in the sequence above: TATA box sequence: (label as TATA) U ATGGCCCTGT AAGCGGTGCG ATGCAATAAA b. What region of mRNA contains the open reading frame that will be translated into protein? Circle one. 51-111 Phenylalanine. (Phe) Leucine (Leu) Leucine (Leu) Isoleucine (Ile) Methionine (Met) 21 61-69 Valine (Val) 71 UCU UCC UCA UCG CCU CCC CCA CCG 121 ACU ACC ACA ACG GCU…
- 1.What is corresponding amino acid chain of the mRNA sequence AUG-CGU-UCU-GCU GGU-UAG? 2. What are the tRNA anticodons of the mRNA codons from item 1? 3. What is the complementary DNA strand of the mRNA strand from item no 1? 4. The DNA strand from item 3 undergoes replication. What will be its complementary DNA strand?10. A portion of 5'-AUGCCACGAGUUGAC-3'. What amino acid sequence does this code for? To answer the question please: I) explain what is the genetic code and list the properties of the genetic e 2) draw a diagram of protein synthesis; 3) determine which tRNA should be attached to the mRNA; 4) what is the anticodon for the very first tRNA that will attach to mRNA? mRNA molecule has the sequence anAre both statements true? 1. Heterogeneous RNA is a term that refers to mRNA that has not been processed. 2. If the %A of a bacteria is 20%, the amount of guanine is 30%. Are both statements true? 1. If the %A of a bacteria is 30%, then the % (G+C) of the bacteria is 40% 2. The DNA is plectonemic because one of the strands go in 5' to 3' direction while the other go in the 3 to 5 direction
- 18. What is a codon? 19. List each codon from the mRNA molecule in #18 (put a dash between each separate codon).Which of the following is not made out of RNA? the carriers that shuffle amino acids to a growing polypeptide strand the ribosome the messenger molecule that provides the code for protein synthesis the intronWhich of the following phases is characterized by preparation for DNA synthesis? G0 G1 G2 S
