Clinical history: A 52-year-old homeless, alcoholic man had a fever and a cough productive of thick sputum that worsened over several days. His temperature is 38.2°C.   Diffuse crackles are heard at the right lung base. Laboratory studies are as follows: hemoglobin: 13.3 g/dL, hematocrit: 40%, platelet count: 291,8000/mm3, WBC count: 13,240/mm3with 71 segmented neutrophils, 7% bands, 16% lymphocytes, and 6% monocytes. Sputum sample stain photo.   What technique would you use to put the sputum sample on the slide? How would you stain the slide? What PPE should you have on while working in the lab? When noting the results above, what would be the correct way to report the results? Looking at the stain, what microbe might be the causative agent?

1. Clinical history: A 52-year-old homeless, alcoholic man had a fever and a cough productive of thick sputum that worsened over several days. His temperature is 38.2°C.   Diffuse crackles are heard at the right lung base. Laboratory studies are as follows: hemoglobin: 13.3 g/dL, hematocrit: 40%, platelet count: 291,8000/mm^3, WBC count: 13,240/mm³with 71 segmented neutrophils, 7% bands, 16% lymphocytes, and 6% monocytes. Sputum sample stain photo.

1. What technique would you use to put the sputum sample on the slide?
2. How would you stain the slide?
3. What PPE should you have on while working in the lab?
4. When noting the results above, what would be the correct way to report the results?
5. Looking at the stain, what microbe might be the causative agent?

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