Chromosome from an A1 cow: 3' 5' Microsatellite 412A100 Beta casein A1 3' 5' A The same locus, but from an A2 cow's chromosome: 3' 5' Microsatellite 412A150 Beta casein A2 3' 5' C a. You have a cow that produces A1 milk and has the following genotype: 412A100 BCA1/ 412A BCA1. This cow mates with a cow that produces A2 milk, the A2 cow's genotype is: 412A150 BCA2/ 412A150 BCA2. 100 i. What is the genotype of the F1? ii. If you copy the microsatellite from the two parent cows and the F1 cow, what banding pattern (DNA fragments) will you see from each sample? Draw the DNA fragments on the sel below romombering that cmaller (chortor) DNA fragments ar found to wardc tho
Chromosome from an A1 cow: 3' 5' Microsatellite 412A100 Beta casein A1 3' 5' A The same locus, but from an A2 cow's chromosome: 3' 5' Microsatellite 412A150 Beta casein A2 3' 5' C a. You have a cow that produces A1 milk and has the following genotype: 412A100 BCA1/ 412A BCA1. This cow mates with a cow that produces A2 milk, the A2 cow's genotype is: 412A150 BCA2/ 412A150 BCA2. 100 i. What is the genotype of the F1? ii. If you copy the microsatellite from the two parent cows and the F1 cow, what banding pattern (DNA fragments) will you see from each sample? Draw the DNA fragments on the sel below romombering that cmaller (chortor) DNA fragments ar found to wardc tho
Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Transcribed Image Text:Chromosome from an A1 cow:
3'
5'
Microsatellite 412A100
Beta casein A1
3'
5'
A
The same locus, but from an A2 cow's chromosome:
3'
5'
Microsatellite 412A150
Beta casein A2
3'
5'
a. You have a cow that produces A1 milk and has the following genotype: 412A100 BCA1/ 412A100
the A2 cow's genotype is: 412A150 BCA2/
cow that produces A2
BCA1. This
412A150 BCA2.
mates with
i.
What is the genotype of the F1?
ii.
If you copy the microsatellite from the two parent cows and the F1 cow, what banding
pattern (DNA fragments) will you see from each sample? Draw the DNA fragments on the
gel below, remembering that smaller (shorter) DNA fragments are found towards the
bottom of the gel compared to longer DNA fragments.
F1 (A1
A1
parent
A2 parent
phenotype)
top
bottom
Transcribed Image Text:4. Examine the figure below showing the location of a microsatellite (called 412A) and the beta casein
gene on chromosome 3, from cows. There are two alleles of the microsatellite that exist in the
population: 412A100 and 412A150, The 100 and 150 represent the number of repeat units in the
microsatellite. There are two alleles of the beta casein gene: BCA1 and BCA2. The two BCA alleles
differ by a single nucleotide in the coding region. The difference results in a change in the protein
such that the beta casein protein from BCA2 is more easily broken-down during digestion, making
the milk more digestible than milk containing BCA1 proteins, for some people.
In the figure below we see a predicted chromosomal map indicating the microsatellite and beta casein
genes. The arrows show the region of DNA that will be copied to assess the microsatellite genotypes.
The bolded "A" and "C" below the beta casein gene shows the nucleotide that differs between the two
alleles.
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