Sample 1 front contains 5,000 of methyl n-amyl ketone and the back section contains 200 µg of methyl n-amyl ketone Sample 2 front contains 4,000 µg of methyl n-amyl ketone and the back section contains 50 µg of methyl n-amyl ketone

This test will differentiate between aromatic amino acids with an acitivated ring from other amino acids. a) Nihydrin Test b) Xanthoproteic Test C) solubility test d) polarimeter test

Using 25 µl of a patient's serum, how much diluent would be needed to create a 1:2 dilution?

Please answer and explain this Biochemsitry Question to the best of your ability

NEORAL oral solution contains 100 mg/mL of cyclosporine. If a pharmacist prepares 30 mL of an oral solution containing 10% w/v cyclosporine, how many milliliters of diluent should be used?

Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…

A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln was applied to column packed with cation-exchange resin at pH 6.0. Which of these dipeptides would be eluted from the column first? O Lys-Gly first O Thr-His first O Ser-Leu first O Glu-Gln first OA mixture of Thr-His and Ser-Leu first

If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the concentration of original protein?

Owing to their structural similarity, separation of mixtures of organic compounds (such as the isomers 1-t- butyl-, 3-t-butyl-, and 4-t-butylcyclohexene) often proves difficult. Silica gel chromatography, however, can be used with some success provided that sufficient silica gel is used to provide optimal separation. Consider, for example, the TLC plate pictured at right after developing and UV visualization where prior to elution, lanes A and B were spotted with 1-t-butylcyclohexene and lanes B and C spotted with 4-t- butylcyclohexene. Which of the following must be true? In the space provided, indicate the CORRECT answers (as 1, 2, and/or 3) then explain your reasoning for all three statements. 1. 1-t-butylcyclohexene elutes faster than 4-t-butylcyclohexene. 2. The R; for both 1-t-butylcyclohexene and 4-t-butylcyclohexene are equal. 3. 4-t-butylcyclohexene is less polar than 1-t-butylcyclohexene

All recipes are given per litre unless specified.   Lambda Broth (LB) Plates                                                  Top Agar   10 g tryptone                                                                           10 g Tryptone 2.5 g NaCl                                                                               2.5 g NaCl 15 g Agar                                                                                7 g Agar   Make 5 mL aliquots, then autoclave.   Lambda Broth (LB) Media                                                  Suspension Media   10 g tryptone                                                                           5.0 g NaCl 2.5 g NaCl                                                                               2 g MgSO4· 7H2O                                                                                                 50 ml 1 M Tris pH 7.5                                                                                                 In LB medium…