Calculate the generation time in a growth experiment in which a medium was inoculated with 5 X 106 cells/mL of E. coli cells and following a 1 hour lag, grew exponentially for 5 hours, after which the population was 5.4 X 109cells/mL

Q: A medium containing an abundance of substrate was seeded with 20 mg/L VSS of an active mixed…

A: Biological culture is a common laboratory method for the study of living things. There are different…

Q: A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had…

A: The number of generations (n) refers to the number of times a cell population got doubled during a…

Q: A batch reactor was initially filled with organic substrate (S0) with an equivalent concentration of…

A: Bioreactor is the equipment used for the production of biomass. Cells consume substrate and multiply…

Q: Consider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and…

A: The given graph represent the biphasic growth of cells. Cells are grown on two carbon sources…

Q: How do we calculate the generation time of a bacterial culture that increases in population of 104…

A: Generation time is defined as the time between two consecutive generations of a population.  With…

Q: You are examining a growth curve data from an experiment. At time 70 min, the optical density was…

A: Bacteria grow very fast if the get the proper medium for their growth and the generation time is the…

Q: What information do you get from these controls to give you confidence in your results? How does…

A: Microorganism are small organism which we can observed through the microscope. It is usually used as…

Q: For the production of a secondary metabolic by Streptomyces coelicolor, a fed batch was performed.…

A: Fed-batch culture is a method of operation in biotechnological processes where one or more nutrients…

Q: A bacteria colony's radius is measured as a function of time. t r(t) 0 1.01 0.1 1.031 Estimate the…

A: In microbiology, the growth rate of a bacterial colony is an important parameter that helps us…

Q: Report the slope and the y-intercept below.  Slope () =                    =     y-intercept () =…

A: In the given graph, an equation is provided. y=-4E-09x + 0.4021R2= 0.1691 The graph represents the…

Q: If  a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine…

A: Bacteria shows exponential growth curve. The growth of the bacteria can be calculated by using the…

Q: Table 6.3 Generation Time Escherichia coli Pseudomonas Lactobacillus Nitrobacter Medium aeruginosa…

A: The growth requirements of bacteria can be understood by observing the generation times in different…

Q: Differential centrifugation can be improved through the use of a density gradient. What is the…

A: In scientific experiments, various techniques are used to isolate, measure or quantify samples based…

Q: E. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a…

A: Microorganisms have complex interactions with their environment and with other microorganisms, and…

Q: Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the…

A: In part (b) of the E. coli growth curve scenario, we're analyzing the potential binding of CAP…

Q: Calculate mean growth rate and generation time of a culture that increase in exponential phase from…

A: Generation time is time taken for culture organisms to double in size. Generation time = t/n  where…

Q: Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. =…

A: In this problem, we have a batch bioreactor containing yeast cells, ribose, and ammonia. The goal is…

Q: Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values…

A: Ks is half velocity constant. S (concentration of substrate) is same. Vmax is same. Ks is inversely…

Q: Explain the results in detail for population growth of E.coli at various temperatures at different…

A: The lower intestine of warm-blooded species frequently holds the bacterium Escherichia coli. It is a…

Q: You have just measured the OD of your E. coli (which is in log-phase growth) and it is at 0.15. You…

A: OD stands for optical density which is the quantitative measure concentration of E. coli cells in…

Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: The growth of bacteria over a period of time is represented by a growth curve. This curve tells the…

Q: Define growth. Why does the lag phase in some bacteria is short or absent and the log phase of the…

A: Grоwth, the inсreаses in сell size аnd number thаt tаke рlасe during the life histоry оf аn оrgаnism…

Q: A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of…

A: A batch bioreactor is a closed device utilized to grow microorganisms for example bacteria, yeast,…

Q: Inoculate 250-μL overnight cell culture into 50 ml LB medium (in a 250 ml flask). Shake vigorously…

A: Cell culture requires specific media according to need of the cells. The media after inoculation is…

Q: Under certain conditions, E. coli cells grow from a concentration of 0.1 to 0.5 kg/m 3 in 1 hour.…

A: Given data:- Initial concentration of bacteria=X = 0.1 kg/m3  Final concentration of bacteria=Xt =…

Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…

A: Answer: E.coli = These are the gram negative bacteria can be found in normal human gut flora.

Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…

Q: Briefly explain, using the bacteria's response to environment, why the time =0 minutes is often not…

A: Bacterial growth has four different phases: Lag phase, log phase, stationary phase, and decline or…

Q: Calculate the colony forming units (CFU) if 100  colonies grow in agar using  a 0.01-mL calibrated…

A: One colony equals to 100 col/mL in  0.01 -mL calibrated loop.

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: What is the equation that represents the mass balance of the exponential phase of bacterial growth?…

A: In exponential growth, a population's per capita (per individual) growth rate stays the same…

Q: An organism produced the following growth pattern when grown at different pH's. The best description…

A: As we know that pH affects both the growth of plants and the activity of plant growth regulators. pH…

Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…

A: Bacteria are microorganisms that grow very fast. They almost double every 20 to 30 minutes.…

Q: Solve for the generation time of the microbe Dextrococcus cemperverens given that the initial number…

A: In this question, we are given: N0 (initial number of microbe present)= 107 N (number of microbes…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: If the number of bacteria were to grow exponentially with a daily rate of r = 0.2612, how long would…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the…

A: Bacterial cells divide by binary fission to produce two daughter cells. The growth of a bacterial…

Q: The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal…

A: There are billions of microorganisms that are present in our surroundings. These microorganisms keep…

Q: Calculate the mean generation time of a bacterium that increased its number from 1 x10° cells/ml to…

A:

Q: Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium:…

A:

Q: In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees…

A: E. coli is a mesophilic bacteria. It functions under certain ideal temperatures and pressure. The…

Q: (b) In a batch bioreactor, the growth of a microbial population is often limited by the availability…

A: As the question contain two different questions, here we have only solved the second one. Bacteria…

Q: Describe how to most easily calculate the generation time for a population of bacteria in a batch…

A: Bacteria grow when one bacterial cell divides to form two daughter cells. Thus, bacterial growth can…