Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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1. Calculate the exact concentration of the stock protein solution in (mg / mL) based on the exact mass of albumin and the volume of the vial used to prepare the broth.

 

 

0.4905 g of albumin in 500 mL volumetric flask (mass of protein used to prepare the stock 

**Concentration of Stock Protein Solution (mg/mL):** 0.4905

**Unknown number:** ___________

**Labquest-SpectroVis Instrument Number:** ___________

| **Sample**        | **Standard Concentration (mg/mL)** | **Absorbance** | **Corrected Absorbance\*** | **Concentration of Diluted Unknown (mg/mL)** |
|-------------------|----------------------------------|----------------|----------------------------|---------------------------------------------|
| Blank             | -------------------------------  | 0.131          | -------------------------  | ------------------------------------------ |
| Standard 1        | -------------------------------  | 0.338          | 0.207                      | ------------------------------------------ |
| Standard 2        | -------------------------------  | 0.568          | 0.237                      | ------------------------------------------ |
| Standard 3        | -------------------------------  | 0.892          | 0.761                      | ------------------------------------------ |
| Standard 4        | -------------------------------  | 0.994          | 0.863                      | ------------------------------------------ |
| Standard 5        | -------------------------------  | 1.138          | 1.007                      | ------------------------------------------ |
| Unknown 1st Replicate | -------------------------------  | 0.421          | 0.290                      | ------------------------------------------ |
| Unknown 2nd Replicate | -------------------------------  | 0.466          | 0.335                      | ------------------------------------------ |
| Unknown 3rd Replicate | -------------------------------  | 0.438          | 0.307                      | ------------------------------------------ |

\*Corrected absorbance is (Absorbance - Blank Absorbance).
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Transcribed Image Text:**Concentration of Stock Protein Solution (mg/mL):** 0.4905 **Unknown number:** ___________ **Labquest-SpectroVis Instrument Number:** ___________ | **Sample** | **Standard Concentration (mg/mL)** | **Absorbance** | **Corrected Absorbance\*** | **Concentration of Diluted Unknown (mg/mL)** | |-------------------|----------------------------------|----------------|----------------------------|---------------------------------------------| | Blank | ------------------------------- | 0.131 | ------------------------- | ------------------------------------------ | | Standard 1 | ------------------------------- | 0.338 | 0.207 | ------------------------------------------ | | Standard 2 | ------------------------------- | 0.568 | 0.237 | ------------------------------------------ | | Standard 3 | ------------------------------- | 0.892 | 0.761 | ------------------------------------------ | | Standard 4 | ------------------------------- | 0.994 | 0.863 | ------------------------------------------ | | Standard 5 | ------------------------------- | 1.138 | 1.007 | ------------------------------------------ | | Unknown 1st Replicate | ------------------------------- | 0.421 | 0.290 | ------------------------------------------ | | Unknown 2nd Replicate | ------------------------------- | 0.466 | 0.335 | ------------------------------------------ | | Unknown 3rd Replicate | ------------------------------- | 0.438 | 0.307 | ------------------------------------------ | \*Corrected absorbance is (Absorbance - Blank Absorbance).
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