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Bleomycin (MM 1500) also known as Blenoxane is an anticancer drug used to treat breast cancer and Hodgkinson's disease. Bleomycin forms a complex with ferrous iron that is believed to represent the active form of the compound. The stoichiometry of the ferrous complex was first determined using the mole-ratio method. Using the data in the table below determine the stoichiometry of the ferrous bleomycin complex. Increasing amounts of 1.01 mM ferrous iron (ferrous sulfate was used) were added to 0.2-mL (how many microliters is this?) aliquots of a solution of bleomycin prepared by the dissolution of 4.5 mg bleomycin in 5-mL of degassed water, pH 7.0 (this means oxygen has been removed, why?). A 60-μL aliquot of each resulting solution was analyzed in a 1-cm micro-cuvet. Volume of added iron, μL Absorbance at 500 nm, A.U. 0.00 25.0 50.0. 75.0 100. 125. 150. 0.0001 0.0430 0.0768 0.1046 0.1278 0.1400 0.1300

Bleomycin (MM 1500) also known as Blenoxane is an anticancer drug used to treat breast cancer and Hodgkinson's disease. Bleomycin forms a complex with ferrous iron that is believed to represent the active form of the compound. The stoichiometry of the ferrous complex was first determined using the mole-ratio method. Using the data in the table below determine the stoichiometry of the ferrous bleomycin complex. Increasing amounts of 1.01 mM ferrous iron (ferrous sulfate was used) were added to 0.2-mL (how many microliters is this?) aliquots of a solution of bleomycin prepared by the dissolution of 4.5 mg bleomycin in 5-mL of degassed water, pH 7.0 (this means oxygen has been removed, why?). A 60-μL aliquot of each resulting solution was analyzed in a 1-cm micro-cuvet. Volume of added iron, μL Absorbance at 500 nm, A.U. 0.00 25.0 50.0. 75.0 100. 125. 150. 0.0001 0.0430 0.0768 0.1046 0.1278 0.1400 0.1300

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter21: Transition Metals And Coordination Chemistry
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Transcribed Image Text:175. 200. 0.1211 0.1145
Bleomycin (MM 1500) also known as Blenoxane is an anticancer drug used to treat breast cancer and Hodgkinson's disease. Bleomycin forms a complex with ferrous iron that is believed to represent the active form of the compound. The stoichiometry of the ferrous complex was first determined using the mole-ratio method. Using the data in the table below determine the stoichiometry of the ferrous bleomycin complex. Increasing amounts of 1.01 mM ferrous iron (ferrous sulfate was used) were added to 0.2-mL (how many microliters is this?) aliquots of a solution of bleomycin prepared by the dissolution of 4.5 mg bleomycin in 5-mL of degassed water, pH 7.0 (this means oxygen has been removed, why?). A 60-μL aliquot of each resulting solution was analyzed in a 1-cm micro-cuvet. Volume of added iron, μL Absorbance at 500 nm, A.U. 0.00 25.0 50.0. 75.0 100. 125. 150. 0.0001 0.0430 0.0768 0.1046 0.1278 0.1400 0.1300
Transcribed Image Text:Bleomycin (MM 1500) also known as Blenoxane is an anticancer drug used to treat breast cancer and Hodgkinson's disease. Bleomycin forms a complex with ferrous iron that is believed to represent the active form of the compound. The stoichiometry of the ferrous complex was first determined using the mole-ratio method. Using the data in the table below determine the stoichiometry of the ferrous bleomycin complex. Increasing amounts of 1.01 mM ferrous iron (ferrous sulfate was used) were added to 0.2-mL (how many microliters is this?) aliquots of a solution of bleomycin prepared by the dissolution of 4.5 mg bleomycin in 5-mL of degassed water, pH 7.0 (this means oxygen has been removed, why?). A 60-μL aliquot of each resulting solution was analyzed in a 1-cm micro-cuvet. Volume of added iron, μL Absorbance at 500 nm, A.U. 0.00 25.0 50.0. 75.0 100. 125. 150. 0.0001 0.0430 0.0768 0.1046 0.1278 0.1400 0.1300
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