Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
Bartleby Related Questions Icon

Related questions

bartleby

Concept explainers

Topic Video
Question
Question 4
Certainly! Here's the transcribed text and descriptions suitable for an educational website:

---

**Transcription of the Educational Text**

4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition:

...Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys...

to the double mutant form:

...Lys-Val-His-Leu-Met-Ala-Ala-Lys...

(a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant.

(b) Which base was added?

(c) Which base was deleted?

---

5. A eukaryotic cell produces a normal protein with the following amino acid sequence: 

Met-Val-His-Lys-Arg-Thr-Leu-Val.

After irradiation, a transformed (mutated) eukaryotic cell is produced that makes a mutant protein from the same coding site on DNA:

Met-Val-His-Lys-Glu-Pro. 

Describe the mutation that occurred. As always, justify your answer.

---

6. You are working with a newly discovered mutagen, and you wish to determine the base change that it introduced into DNA. Thus far you have determined that the mutagen chemically alters a single base in the DNA but this base-pairing properties are altered permanently. In order to determine the specificity of the alteration, you examine the amino acid changes that place after mutagenesis. A sample of what you find is shown below:

Original: Gln-His-Ile-Glu-Lys  
Mutant: Gln-His-Met-Glu-Lys

Original: Ala-Val-Asn-Arg  
Mutant: Ala-Val-Ser-Arg

---

In addition to the textual information, the document features a keyboard image without any specific educational relevance. The focus should be on the educational content described.
expand button
Transcribed Image Text:Certainly! Here's the transcribed text and descriptions suitable for an educational website: --- **Transcription of the Educational Text** 4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition: ...Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys... to the double mutant form: ...Lys-Val-His-Leu-Met-Ala-Ala-Lys... (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant. (b) Which base was added? (c) Which base was deleted? --- 5. A eukaryotic cell produces a normal protein with the following amino acid sequence: Met-Val-His-Lys-Arg-Thr-Leu-Val. After irradiation, a transformed (mutated) eukaryotic cell is produced that makes a mutant protein from the same coding site on DNA: Met-Val-His-Lys-Glu-Pro. Describe the mutation that occurred. As always, justify your answer. --- 6. You are working with a newly discovered mutagen, and you wish to determine the base change that it introduced into DNA. Thus far you have determined that the mutagen chemically alters a single base in the DNA but this base-pairing properties are altered permanently. In order to determine the specificity of the alteration, you examine the amino acid changes that place after mutagenesis. A sample of what you find is shown below: Original: Gln-His-Ile-Glu-Lys Mutant: Gln-His-Met-Glu-Lys Original: Ala-Val-Asn-Arg Mutant: Ala-Val-Ser-Arg --- In addition to the textual information, the document features a keyboard image without any specific educational relevance. The focus should be on the educational content described.
Expert Solution
Check Mark
Step 1

4. The wild type     Lys-ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys

The double mutant    Lys-Val-His-His-Leu-Met-Ala-Ala-Lys

Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Text book image
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Text book image
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Text book image
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Text book image
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education