[S] mM 0.0001 0.0002 0.0005 0.001 0.002 0.005 0.01 0.02 0.05 0.1 12 Vo Without inhibitor (mM/min) 34 50 71 83 91 96 198 99 100 100 100 B. 0.0002 μM Vo With inhibitor (mM/min) M. Competitive N. Non-competitive O. Uncompetitive P. Allosteric Q. Mixed 17 29 50 DC. 2.0 μM DD. 0.01 μM/min DE. 0.0005 μM F. CG. The rate is doubled CH. The rate is reduced by 25% 1. The rate remains the same J. The rate is halved K. The rate increases by a factor of 5 67 Part A: What is the apparent KM of substrate enzyme binding in the presence of inhibitor? (Choose from options A- E) --:::::: Part B: If an experiment is performed using 0.0001 μM of substrate once in the absence of inhibitor and once in the presence of inhibitor, what effect will the inhibitor have on the reaction rate? (Choose from options G-K) 99 Part C: Without plotting the data, what type of inhibitor was used in this experiment? (Choose from options M-Q) DA. 2.0 µM/min
[S] mM 0.0001 0.0002 0.0005 0.001 0.002 0.005 0.01 0.02 0.05 0.1 12 Vo Without inhibitor (mM/min) 34 50 71 83 91 96 198 99 100 100 100 B. 0.0002 μM Vo With inhibitor (mM/min) M. Competitive N. Non-competitive O. Uncompetitive P. Allosteric Q. Mixed 17 29 50 DC. 2.0 μM DD. 0.01 μM/min DE. 0.0005 μM F. CG. The rate is doubled CH. The rate is reduced by 25% 1. The rate remains the same J. The rate is halved K. The rate increases by a factor of 5 67 Part A: What is the apparent KM of substrate enzyme binding in the presence of inhibitor? (Choose from options A- E) --:::::: Part B: If an experiment is performed using 0.0001 μM of substrate once in the absence of inhibitor and once in the presence of inhibitor, what effect will the inhibitor have on the reaction rate? (Choose from options G-K) 99 Part C: Without plotting the data, what type of inhibitor was used in this experiment? (Choose from options M-Q) DA. 2.0 µM/min
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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