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Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Q27
Please solve all u can
![[S] mM
0.0001
0.0002
0.0005
0.001
0.002
0.005
0.01
0.02
0.05
0.1
12
Vo Without inhibitor
(mM/min)
34
50
71
83
91
96
198
99
100
100
100
B. 0.0002 μM
Vo With inhibitor
(mM/min)
M. Competitive
N. Non-competitive
O. Uncompetitive
P. Allosteric
Q. Mixed
17
29
50
DC. 2.0 μM
DD. 0.01 μM/min
DE. 0.0005 μM
F.
CG. The rate is doubled
CH. The rate is reduced by 25%
1. The rate remains the same
J. The rate is halved
K. The rate increases by a factor of 5
67
Part A: What is the apparent KM of substrate enzyme binding in the presence of inhibitor? (Choose from options A-
E)
--::::::
Part B: If an experiment is performed using 0.0001 μM of substrate once in the absence of inhibitor and once in the
presence of inhibitor, what effect will the inhibitor have on the reaction rate? (Choose from options G-K)
99
Part C: Without plotting the data, what type of inhibitor was used in this experiment? (Choose from options M-Q)
DA. 2.0 µM/min](https://content.bartleby.com/qna-images/question/2408fde7-061f-4d4e-ab1d-efb83159d466/540b47b2-7917-4ae7-970f-97fd9adbafa4/xpgk44xi_thumbnail.jpeg)
Transcribed Image Text:[S] mM
0.0001
0.0002
0.0005
0.001
0.002
0.005
0.01
0.02
0.05
0.1
12
Vo Without inhibitor
(mM/min)
34
50
71
83
91
96
198
99
100
100
100
B. 0.0002 μM
Vo With inhibitor
(mM/min)
M. Competitive
N. Non-competitive
O. Uncompetitive
P. Allosteric
Q. Mixed
17
29
50
DC. 2.0 μM
DD. 0.01 μM/min
DE. 0.0005 μM
F.
CG. The rate is doubled
CH. The rate is reduced by 25%
1. The rate remains the same
J. The rate is halved
K. The rate increases by a factor of 5
67
Part A: What is the apparent KM of substrate enzyme binding in the presence of inhibitor? (Choose from options A-
E)
--::::::
Part B: If an experiment is performed using 0.0001 μM of substrate once in the absence of inhibitor and once in the
presence of inhibitor, what effect will the inhibitor have on the reaction rate? (Choose from options G-K)
99
Part C: Without plotting the data, what type of inhibitor was used in this experiment? (Choose from options M-Q)
DA. 2.0 µM/min
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