Benesi-Hildebrand Plot.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
icon
Related questions
Question
I need the answer as soon as possible
Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs.
1
X-
(F-F0) Ka(Foo-F0) [CD]° (Foo-F0)
1
1/[CD]0 (x-axis). Fit the data to Equation
The fitting parameters of the data to a straight-line equation (y = mx + b)
should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting
the Ka value.
[ME-b-CD]
X ul of ME-b-CD
ul of ANS
Y ul of
Fluorescence
(mM)
(20mM)
(0.4mM)
PBS
[CD] mM
intensity
F- FO
0.
500
1500
0.
0.37
1
100
500
1400
9.426
9.056
200
500
1300
2.
16.76
16.39
3
300
500
1200
3.
22.621
22.251
4.
400
500
1100
4.
27.496
27.126
.500
500
1000
5.
30.573
30.203
6.
600
500
900
6.
34.344
33.974
700
500
800
7.
36.991
36.621
8.
800
500
700
8.
38.478
38.108
900
500
600
9.
41.012
40.642
10
1000
500
500
10
43.769
43.399
12
1200
500
300
12
45.64
45.27
14
1400
500
100
14
47.52
47.15
15
1500
500
15
48.359
47.989
Transcribed Image Text:Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs. 1 X- (F-F0) Ka(Foo-F0) [CD]° (Foo-F0) 1 1/[CD]0 (x-axis). Fit the data to Equation The fitting parameters of the data to a straight-line equation (y = mx + b) should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting the Ka value. [ME-b-CD] X ul of ME-b-CD ul of ANS Y ul of Fluorescence (mM) (20mM) (0.4mM) PBS [CD] mM intensity F- FO 0. 500 1500 0. 0.37 1 100 500 1400 9.426 9.056 200 500 1300 2. 16.76 16.39 3 300 500 1200 3. 22.621 22.251 4. 400 500 1100 4. 27.496 27.126 .500 500 1000 5. 30.573 30.203 6. 600 500 900 6. 34.344 33.974 700 500 800 7. 36.991 36.621 8. 800 500 700 8. 38.478 38.108 900 500 600 9. 41.012 40.642 10 1000 500 500 10 43.769 43.399 12 1200 500 300 12 45.64 45.27 14 1400 500 100 14 47.52 47.15 15 1500 500 15 48.359 47.989
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Knowledge Booster
Temperature and heat storage
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemical-engineering and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The