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B. Color Reactions Test Biuret test Samples Added reagent/s Observation and Interpretation

B. Color Reactions Test Biuret test Samples Added reagent/s Observation and Interpretation

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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B. Color Reactions Test Biuret test Hopkins-Cole test Millon's test Reduces Sulfur test Samples Added reagent/s Observation and Interpretation
Transcribed Image Text:B. Color Reactions Test Biuret test Hopkins-Cole test Millon's test Reduces Sulfur test Samples Added reagent/s Observation and Interpretation
B. Color Reactions (Use 2mL of the Protein solution) 1. Biuret test - Mix 2mL of egg albumin solution with 1mL of dilute NaOH and 10 drops of diluted CuSO4 solution in a test tube. Shake well. Note the change in color. 2. Millon's test - To 2mL egg albumin, add 10 drops of Millon's reagent. Shake well and heat over a water bath to boiling. Note the color change. 3. Hopkins-Cole test - To 5 drops of egg albumin solution, add 5 drops of glyoxylic acid. Carefully run down 5 drops of conc. H₂SO4 on the side of the test tube. Note the color formed at the point of contact between the two liquids. 4. Reduces Sulfur test - To 2mL of egg albumin solution in a test tube, add 1mL of 20% NaOH and 10 drops of 10% Lead acetate solution. Cover the test tube with a cork and boil in a water bath for two minutes. Observe the color change and explain.
Transcribed Image Text:B. Color Reactions (Use 2mL of the Protein solution) 1. Biuret test - Mix 2mL of egg albumin solution with 1mL of dilute NaOH and 10 drops of diluted CuSO4 solution in a test tube. Shake well. Note the change in color. 2. Millon's test - To 2mL egg albumin, add 10 drops of Millon's reagent. Shake well and heat over a water bath to boiling. Note the color change. 3. Hopkins-Cole test - To 5 drops of egg albumin solution, add 5 drops of glyoxylic acid. Carefully run down 5 drops of conc. H₂SO4 on the side of the test tube. Note the color formed at the point of contact between the two liquids. 4. Reduces Sulfur test - To 2mL of egg albumin solution in a test tube, add 1mL of 20% NaOH and 10 drops of 10% Lead acetate solution. Cover the test tube with a cork and boil in a water bath for two minutes. Observe the color change and explain.
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