b) The methyl group at the C terminus of aspartame can be cleaved by concentrated sulfuric acid (conc. H₂SO4) to reveal the carboxylic acid (compound 1), which has a pK₂ of 2.43. OH NH₂ conc. OCH3 H₂SO4 OH NH₂ OH Compound 1 The other two pKa values in the molecule are unchanged. It is useful to calculate the isoelectric point (pl) when planning to separate charged species from neutral species with electrophoresis. pKal + PK₂2 pl = 2 HINT: i) Calculate the isoelectric point of aspartame. ii) Calculate the isoelectric point of compound 1 and explain your reasoning behind the calculation.

Guanidine and the guanidino group present in arginine are two of the strongest organic bases known. Account for their basicity.

Trypsin is a serine protease that has a KM of 15 mM. At an initial substrate concentration of 250 µM insulin, trypsin is found to degrade insulin at a rate of 0.6 µM/min. If the concentration of trypsin in solution was 4.326 µM, what is the kcat of trypsin in s-1?

A biochemist completely digests a glycerophospholipid with a mixture of phospholipases A and D. HPLC and mass spectrometry analysis reveals the presence of an amino acid of 105,09 Da, a saturated fatty acid of 256.43 Da, and an omega-3 monounsaturated fatty acid of 282.45 Da. Which amino acid does the glycerophospholipid contain? O valine (C,H, NO,) O alanine (C, H,NO,) O scrine (C,H,NO,) O proline (C,H,NO,) Modify the phosphoglycerol backbone to draw the most likely structure of the lipid. Add the amino acid using the groups tool. Draw Rings Groups More Erase Select

Glutamic acid has three protonatable groups (two carboxyls and one amino). Calculate its isoelectric point, indicating what values ​​of pKa you used to calculate it.

The monoanion of adenosine monophosphate (AMP) is an intermediate in phosphate metabolism: А—О—р—он АMP—ОН- where A = adenosine. If the pKa for this anion is 7.21, what is the ratio of [AMP-OH ] to [AMP-02 ]in blood at pH 7.4?