As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation

Differential interference microscopy needs many optical parts, thus it may be terribly costly to line up. light from an incandescent supply is responded to a polarizer, in order that all of the light obtaining through should vibrate in a very single plane. The beam is then responded to a prism that separates it into elements that are separated by a really little distance - capable the resolution of the target lens. The beams meet up with the condenser, then the specimen.