Are there specific E. coli strains used to make competent cells? Explain the characteristics of these strains

You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]

Given that antibiotic-resistant strains of bacteria are a concern to the general health of a population, what can be done to prevent their development?

Under what circumstances can E. coli cause disease and what delivers the gene/plasmid?

What is a plasmid and how does it contribute to the spread of antibiotic resistance?

How do Restriction Enzymes like EcoRI work?

In Methicillin resistant bacteria, what have the bacteria developed that allows them to stay alive in the presence of Methicillin?

Give two reasons why E. coli is naturally resistant to antibiotics like Penicillin?

List two methods that can be used to make E. coli cells competent. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA er T? v

Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2