Anti-A Anti-B Anti-Rh Blood Type Slide #1: Mr. Smith Agglutination No Agglutination Agglutination A+ Slide #2: Ms. Jones No agglutination Agglutination No agglutination B- Slide #3: Mr. Green Agglutination Agglutination Agglutination AB+ Slide #4: Ms. Brown No agglutination No agglutination No agglutination O- What ABO antigens are present on the red blood cells of Mr. Green’s blood? What ABO antibodies are present in the plasma of Mr. Green’s blood?

Anti-A Anti-B Anti-Rh Blood Type Slide #1: Mr. Smith Agglutination No Agglutination Agglutination A+ Slide #2: Ms. Jones No agglutination Agglutination No agglutination B- Slide #3: Mr. Green Agglutination Agglutination Agglutination AB+ Slide #4: Ms. Brown No agglutination No agglutination No agglutination O- What ABO antigens are present on the red blood cells of Mr. Green’s blood? What ABO antibodies are present in the plasma of Mr. Green’s blood?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.3: Microhematocrit

Problem 4RQ

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What would happen if someone did not receive a compatible blood type during a blood transfusion? (Hint: Reaction between antigens and antibodies when blood typing.)
Table 4: Presence or Absence of Agglutination Mother 1 Father 1 Child 1 Mother 2 Father 2 Child 2 Mother 3 Father 3 Child 3 Anti-A Sera Agglut No Agglut No No No Agglut No Agglut Anti-B Sera Agglut No No No Agglut Agglut No No No Anti-Rh Sera No Agglut Agglut Agglut No Agglut No No No Blood Type AB- 0+ A+ O+ B- B+ A- O- А- What is the blood type of the mother, father, and child in Family 1? What is the blood type of the mother, father, and child in Family 2? What is the blood type of the mother, father, and child in Family 3?
Phenotype: A2B 1. Give the possible genotype/s of A:B 2. If you were to perform the blood typing of this individual, what are the expected results given the following reagents Phenotype Forward typing (reagents) Reverse typing (reagents) Anti-A Anti-B Anti-H A cells B cells Al Write "+" for presence of agglutination and "-" for absence of agglutination 3. Explain the results of the blood typing, why would there be a "+" or a "" reaction during the testing. Relate your answer to the presence/absence of antigens and/or antibodies
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Table 4: Presence or Absence of Agglutination Child 1 Mother 3 Mother 2 Father 2 No Child 2 No Anti-A Sera No Agglut Agglut No Anti-B Sera No Agglut Agglut No Anti-Rh Sera Agglut Agglut No Agglut No Blood Type AB- O+ ▼ A+ O+ B- ▼ B+ ▼ What is the blood type of the mother, father, and child in Family 1? What is the blood type of the mother, father, and child in Family 2? What is the blood type of the mother, father, and child in Family 3? Mother 1 Father 1 Agglut No Agglut No No Agglut Father 3 No No No O- Child 3 Agglut No No A- ▼
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Rewrite the following sentences after correction. The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B. Indirect ELISA can detect polygenic gene expression.
Table 4: Presence or Absence of Agglutination Mother 1 Father 1 Child 1 Mother 2 Father 2 Child 2 Mother 3 Father 3 Child 3 Anti-A Sera Agglut No Agglut No No No Agglut No Agglut Anti-B Sera Agglut No No No Agglut Agglut No No No Anti-Rh Sera No Agglut Agglut Agglut No Agglut No No No Select: Select: Select: Select: Select: Select: Select: Select: Select: Blood Type
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Rewrite the following sentences after correction. Indirect ELISA can detect polygenic gene expression.
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	Anti-A	Anti-B	Anti-Rh	Blood Type

Slide #1: Mr. Smith	Agglutination	No Agglutination	Agglutination	A+
Slide #2: Ms. Jones	No agglutination	Agglutination	No agglutination	B-
Slide #3: Mr. Green	Agglutination	Agglutination	Agglutination	AB+
Slide #4: Ms. Brown	No agglutination	No agglutination	No agglutination	O-