Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
Bartleby Related Questions Icon

Related questions

Question
100%

Explain this problem

Answer all questions
Q1) The chromatogram below show a separation of a mixture of 2 components injected in a 25
cm column at a flow rate of 0.4 mlmin.
16
12
B
20
30
40
Time, min
10
50
60
70
A chromatogram of a two-component mixture
Answer the following questions :
a. Find the times that components A and B spend in the stationary phase.
b. Find the retention times for A and B.
c. Determine the retention factors for the two components.
d. Find the full widths of each peak and the full width at half maximum values.
e. Find the resolution of the two peaks.
f. Find the average number of plates for the column.
g. Find the average plate height.
h. What column length would be needed to achieve a resolution of 1.75?
i. What time would be required to achieve the resolution in part h.
j. Assume that the column length is fixed at 25 cm and the packing material is fixed. What
measures
could you take to increase the resolution to achieve baseline separation?
k. Are there any measures you could use to achieve a better separation in a shorter time with
the same
column as in part j?
14
10
4.
2.
Detector response
expand button
Transcribed Image Text:Answer all questions Q1) The chromatogram below show a separation of a mixture of 2 components injected in a 25 cm column at a flow rate of 0.4 mlmin. 16 12 B 20 30 40 Time, min 10 50 60 70 A chromatogram of a two-component mixture Answer the following questions : a. Find the times that components A and B spend in the stationary phase. b. Find the retention times for A and B. c. Determine the retention factors for the two components. d. Find the full widths of each peak and the full width at half maximum values. e. Find the resolution of the two peaks. f. Find the average number of plates for the column. g. Find the average plate height. h. What column length would be needed to achieve a resolution of 1.75? i. What time would be required to achieve the resolution in part h. j. Assume that the column length is fixed at 25 cm and the packing material is fixed. What measures could you take to increase the resolution to achieve baseline separation? k. Are there any measures you could use to achieve a better separation in a shorter time with the same column as in part j? 14 10 4. 2. Detector response
Expert Solution
Check Mark
Knowledge Booster
Background pattern image
Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Text book image
Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry
Chemistry
ISBN:9781259911156
Author:Raymond Chang Dr., Jason Overby Professor
Publisher:McGraw-Hill Education
Text book image
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning
Text book image
Organic Chemistry
Chemistry
ISBN:9780078021558
Author:Janice Gorzynski Smith Dr.
Publisher:McGraw-Hill Education
Text book image
Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning
Text book image
Elementary Principles of Chemical Processes, Bind...
Chemistry
ISBN:9781118431221
Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY