Answer all questions Q1) The chromatogram below show a separation of a mixture of 2 components injected in a 25 cm column at a flow rate of 0.4 mlmin. 16 12 B 20 30 40 Time, min 10 50 60 70 A chromatogram of a two-component mixture Answer the following questions : a. Find the times that components A and B spend in the stationary phase. b. Find the retention times for A and B. c. Determine the retention factors for the two components. d. Find the full widths of each peak and the full width at half maximum values. e. Find the resolution of the two peaks. f. Find the average number of plates for the column. g. Find the average plate height. h. What column length would be needed to achieve a resolution of 1.75? i. What time would be required to achieve the resolution in part h. j. Assume that the column length is fixed at 25 cm and the packing material is fixed. What measures could you take to increase the resolution to achieve baseline separation? k. Are there any measures you could use to achieve a better separation in a shorter time with the same column as in part j? 14 10 4. 2. Detector response

Answer all questions Q1) The chromatogram below show a separation of a mixture of 2 components injected in a 25 cm column at a flow rate of 0.4 mlmin. 16 12 B 20 30 40 Time, min 10 50 60 70 A chromatogram of a two-component mixture Answer the following questions : a. Find the times that components A and B spend in the stationary phase. b. Find the retention times for A and B. c. Determine the retention factors for the two components. d. Find the full widths of each peak and the full width at half maximum values. e. Find the resolution of the two peaks. f. Find the average number of plates for the column. g. Find the average plate height. h. What column length would be needed to achieve a resolution of 1.75? i. What time would be required to achieve the resolution in part h. j. Assume that the column length is fixed at 25 cm and the packing material is fixed. What measures could you take to increase the resolution to achieve baseline separation? k. Are there any measures you could use to achieve a better separation in a shorter time with the same column as in part j? 14 10 4. 2. Detector response

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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