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Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Question
An unknown mixture was analyzed with TLC on a silica gel plate. Development gave two spots with Rf values of 1.0 and 0.0 respectively.
a) Can you say for sure how many compounds are present in the mixture? If so, how many are there? Explain.
b) If not, what additional TLC experiment(s) might be performed to determine how many components are present? Explain.
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- Follow-up questions 1. What is the purpose of the lid on the colorimeter? 2. In part 1, why is it necessary to calibrate absorbance at every wavelength? 3. In part 1, the concentration of the CV+ stock solution was 12.5 uM. What mass of CV+ is found in 1 liter of this solution? Give your answer in milligrams. (The molar mass of crystal violet is 407.979g.) 6. 4. What are the effects of the following changes on molar absorptivity? (a) when the concentration of CV+ changes? (b) during the course of the color-loss reaction? (c) when absorbance is measured at different wavelengths? 5. In part 2, what labware or apparatus would you use to prepare solutions for the standard curve? In part 3, what percentage of the NaOH is left at the end of the color-loss reaction, assuming all the crystal violet has reacted? Show your calculation. 6. 7. (a) In part 3, how long is the half-life of the color-loss reaction (show the calculation)? (b) In part 4, is the half-life of the color-loss reaction…arrow_forward2) Consider the following compounds. Which rankings list these compounds in terms of Rf from lowest to highest using a silica gel thin layer chromatography plate and 1:1 Ethyl Acetate:Hexanes as the developing solvent. (e. g. Aarrow_forward
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