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An HPLC setup has an injection volume of 0.495 mL, a detector volume of 0.305 ml, and contains 32.0 cm of tubing, which has a diameter of 0.085 cm. The baseline width, twobs, of a band eluting from the column at a flow rate of 1.61 ml/min is observed to be 0.791 min. Assuming a diffusion coefficient of 7.2 x 10-9 m2/s, calculate the baseline width of the band, in minutes, if the column was the only source of broadening. 8 x10-4 Weolumn min Incorrect
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Transcribed Image Text:An HPLC setup has an injection volume of 0.495 mL, a detector volume of 0.305 ml, and contains 32.0 cm of tubing, which has a diameter of 0.085 cm. The baseline width, twobs, of a band eluting from the column at a flow rate of 1.61 ml/min is observed to be 0.791 min. Assuming a diffusion coefficient of 7.2 x 10-9 m2/s, calculate the baseline width of the band, in minutes, if the column was the only source of broadening. 8 x10-4 Weolumn min Incorrect
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