An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC. The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?
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An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC. The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?
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- Can a tablet be sufficient in neutralizing a 100 mL of stomach acid with a pH of 1.50? Hint: H+ concentration is antilog(-pH). Answer it by YES or NO, why?Consider a mixture comprised of the proteins attached: Protein that will most strongly bind to an anion exchange column? Protein that will elute first in gel filtration chromatography? Protein that will elute last in hydrophobic interaction chromatography? Protein that will elute last in carbohydrate containing column?Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > Glycine You are trying to separate a mixture of AMP, ADP, and ATP using ion exchange chromatography. Select appropriate supplies for your experiment. A positively charged column, deprotonated buffer, NaCl of same concentration. A positively charged column, protonated buffer, NaCl of increasing concentration. A negatively charged column, protonated buffer, NaCl of increasing concentration You cannot separate nucleotides using ion exchange chromatography.