Amino acid Histidine Glycine Aspartate pka values 1.8 6.0 9.2 2.3 9.6 2.1 3.9 9.8 T.A. pl value Ivom Net Charge @pH 4.4 Net Charge @pH 13 Relative expected time of elution from chromatography column (1st/2nd/3rd) T.A.
Amino acid Histidine Glycine Aspartate pka values 1.8 6.0 9.2 2.3 9.6 2.1 3.9 9.8 T.A. pl value Ivom Net Charge @pH 4.4 Net Charge @pH 13 Relative expected time of elution from chromatography column (1st/2nd/3rd) T.A.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Fill out the related information for all three amino acids in the chart. On a separate piece of paper provide calculations for all pl values; one a-amino group; one a-carboxyl group and all the ionizable R-groups. pKa table provided.
pH = pKa + log (cb/ca)
Transcribed Image Text:Amino Acid Abbreviation
Alanine
ala
Arginine
arg
Asparagine asn
Aspartate/
asp
Aspartic Acid
Cysteine
cys
Glutamate/ glu
Glutamic Acid
Glutamine gln
gly
his
Ile
leu
lys
Methionine met
Phenylalanine phe
pro
ser
thr
Glycine
Histidine
Isoleucine
Leucine
Lysine
Proline
Serine
Threonine
Tryptophan trp
Tyrosine
tyr
Valine
val
pK₁
pK₂
PKR
(a-carboxyl) (a-amino) (side chain)
2.3
2.2
2.0
2.1
1.7
2.2
2.2
2.3
1.8
2.4
2.4
2.2
2.3
1.8
2.0
2.2
2.6
2.4
2.2
2.3
9.7
9.0
8.8
9.8
10.8
9.7
9.1
9.6
9.2
9.7
9.6
9.0
9.2
9.1
10.6
9.2
10.4
9.4
9.1
9.6
12.5
3.9
8.3
4.3
6.0
10.5
10.1
Transcribed Image Text:Amino acid
Histidine
Glycine
Aspartate
pka
values
1.8
6.0
9.2
2.3
9.6
2.1
3.9
9.8
T.A.
pl value
Net Charge
@pH 4.4
Net Charge
@pH 13
Relative expected
time of elution from
chromatography
column (1st/2nd/3rd)
T.A.
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