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(ABOUT MICROSCOPES)
Describe the relationship between field of view and magnification.
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- Explain which microscope, electron or light, has greater resolving power and explain why.A lab revolving around compound microscopes was conducted. Please answer the following questions (questions 1, 2, and 3). If a specimen (placed on a slide) has little or no color, what level of light intensity should you use; should the intenisty of the light be high or low (choose)? Which objective should be in position before you start to focus the microscope? Which focus knob should you use first with this objective?What is the importance of using an immersion oil in 100x magnification? Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. Give some limitations in the use of microscope. Conclusion about microscope
- make a comparison viewing a microscope slide under low magnification and high magnification by identifying benefits and drawbacks for each.Alternatives used on a light microscope might improve vision by using a different light source. Name two types of light sources.How can microscopes be classified according to the number of eyepieces?
- a. When buying a microscope, what features are most important tocheck for?b. What is probably true of a $20 microscope that claims to magnify1,000×?Demonstrate how to view an object with the microscope using all magnificationsCareless Kris is using the microscope for the first time to look at cells and breaks a slide at high power. Explain to Kris the correct order of which objects lenses and focus knobs to use at each step when focusing a microscope from start to finish (hgih power).
- What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.The magnification possible with any type of microscope is an important property, but its resolution, the ability to distinguish between two very closely apposed objects, is even more critical. Describe why the resolving power of a microscope is more important for seeing finer details than its magnification.Based on your observations: Please answer in a full sentence. 1. At what magnification you can see the largest area on your slide? 2. At what magnification you can see the smallest FOV on the slide? 3. What happens to the light intensity as you increase the magnification? 4. What is the proper way to focus a microscope?