Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN: 9781259696527
Author: J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher: McGraw-Hill Education
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1. Determination of Kinetic Constants in an Inhibited Enzyme Reaction. Biological oxidation of phenylacetic acid is inhibited by propionic acid present in wastewater. The following data were obtained for enzymatic oxidation of phenylacetic acid at different concentrations of propionic acid: a. What kind of inhibition is this? b. Determine Vmax, Km, and KI? Table 1 S (MM) 0.25 V (mM/1*hr), I = 0 mm 1.0 V (mM/1*hr), I = 1 mM 0.65 V (mM/1*hr), I = 2 mM | 0.55 *mM = millimolar 0.40 1.7 1.1 0.9 0.50 1.9 1.3 1.0 0.60 2.1 1.4 1.3 0.75 2.4 1.8 1.4 1.00 2.5 2.2 1.8
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Transcribed Image Text:1. Determination of Kinetic Constants in an Inhibited Enzyme Reaction. Biological oxidation of phenylacetic acid is inhibited by propionic acid present in wastewater. The following data were obtained for enzymatic oxidation of phenylacetic acid at different concentrations of propionic acid: a. What kind of inhibition is this? b. Determine Vmax, Km, and KI? Table 1 S (MM) 0.25 V (mM/1*hr), I = 0 mm 1.0 V (mM/1*hr), I = 1 mM 0.65 V (mM/1*hr), I = 2 mM | 0.55 *mM = millimolar 0.40 1.7 1.1 0.9 0.50 1.9 1.3 1.0 0.60 2.1 1.4 1.3 0.75 2.4 1.8 1.4 1.00 2.5 2.2 1.8
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