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A. Unknown (a solution where the amount of protein is not known): Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of unknown protein sample (UK). Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes of water and the P20 for volumes of the stock). Be sure to understand what the dilution factor is for each sample (dilution factor = the number used to determine the actual concentration of the stock UK after the diluted samples are analyzed). Table #1 Generation of Serial Dilutions Sample ID 1:50 1:100 1:250 Water 490 ul 495 ul 498 μl Unknown Stock 10 ul 5 μl 2 μl

A. Unknown (a solution where the amount of protein is not known): Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of unknown protein sample (UK). Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes of water and the P20 for volumes of the stock). Be sure to understand what the dilution factor is for each sample (dilution factor = the number used to determine the actual concentration of the stock UK after the diluted samples are analyzed). Table #1 Generation of Serial Dilutions Sample ID 1:50 1:100 1:250 Water 490 ul 495 ul 498 μl Unknown Stock 10 ul 5 μl 2 μl

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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. Be sure to understand what the dilution
factor is for each sample (dilution factor = the number used to determine the actual
concentration of the stock UK after the diluted samples are analyzed) ?

A. Unknown (a solution where the amount of protein is not known): Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of unknown protein sample (UK). Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes of water and the P20 for volumes of the stock). Be sure to understand what the dilution factor is for each sample (dilution factor = the number used to determine the actual concentration of the stock UK after the diluted samples are analyzed). Table #1 - Generation of Serial Dilutions Sample ID 1:50 1:100 1:250 Water 490 ul 495 μl 498 ul Unknown Stock 10 μl 5 μl 2 μl Close the cap on each tube and vortex to mix (5-10 sec). There will now be four samples of the unknown at different concentrations (the stock UK is the fourth sample). The four samples will now be used in the BCA assay.
Transcribed Image Text:A. Unknown (a solution where the amount of protein is not known): Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of unknown protein sample (UK). Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes of water and the P20 for volumes of the stock). Be sure to understand what the dilution factor is for each sample (dilution factor = the number used to determine the actual concentration of the stock UK after the diluted samples are analyzed). Table #1 - Generation of Serial Dilutions Sample ID 1:50 1:100 1:250 Water 490 ul 495 μl 498 ul Unknown Stock 10 μl 5 μl 2 μl Close the cap on each tube and vortex to mix (5-10 sec). There will now be four samples of the unknown at different concentrations (the stock UK is the fourth sample). The four samples will now be used in the BCA assay.
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