Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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. Be sure to understand what the dilution
factor is for each sample (dilution factor = the number used to determine the actual
concentration of the stock UK after the diluted samples are analyzed) ?

A. Unknown (a solution where the amount of protein is not known):
Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of
unknown protein sample (UK).
Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes
of water and the P20 for volumes of the stock). Be sure to understand what the dilution
factor is for each sample (dilution factor = the number used to determine the actual
concentration of the stock UK after the diluted samples are analyzed).
Table #1 - Generation of Serial Dilutions
Sample ID
1:50
1:100
1:250
Water
490 ul
495 μl
498 ul
Unknown Stock
10 μl
5 μl
2 μl
Close the cap on each tube and vortex to mix (5-10 sec). There will now be four samples of
the unknown at different concentrations (the stock UK is the fourth sample). The four
samples will now be used in the BCA assay.
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Transcribed Image Text:A. Unknown (a solution where the amount of protein is not known): Obtain three microfuge tubes. Label them 1:50, 1:100 and 1:250. Obtain a 40 μl stock of unknown protein sample (UK). Create dilutions of the UK sample based upon Table 1 (use the P1000 to transfer volumes of water and the P20 for volumes of the stock). Be sure to understand what the dilution factor is for each sample (dilution factor = the number used to determine the actual concentration of the stock UK after the diluted samples are analyzed). Table #1 - Generation of Serial Dilutions Sample ID 1:50 1:100 1:250 Water 490 ul 495 μl 498 ul Unknown Stock 10 μl 5 μl 2 μl Close the cap on each tube and vortex to mix (5-10 sec). There will now be four samples of the unknown at different concentrations (the stock UK is the fourth sample). The four samples will now be used in the BCA assay.
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