a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope.
a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Answer question a and b, especially b. Draw what is needed in the question. Use the 2nd image as reference
Transcribed Image Text:Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently
photobleached. The recovery of fluorescence was monitored and gave the following profile:
100
M
S
time
a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of
your ranking based on their FRAP curves and the most probable appearance and composition
of the three membranes.
b. Draw the expected image of the surface of the three membranes before photobleaching
and after fluorescence recovery as viewed under a fluorescence microscope.
% fluorescence
Transcribed Image Text:FRAP (Fluorescence Recovery After Photobleaching)
(A) 1
Immobile fraction
Photoech
2
3
Recovery
half time
Time
Bleach
6
Fluorescence intensity in
bleach ROI
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