A series of standard calibration solutions were prepared in 100 mL volumetric flask by reacting standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce the characteristic blue color. The concentration of stock solution was 100 mg/L. The absorbance, A were measured using colorimetric spectrometer as shown in the below table. A calibration curve was plotted against the concentration of phosphorous. Volume of Phosphorous from stock solution (mL) Concentration of Absorbance phosphorus (mg/L) 1 0.001 5 0.086 10 0.173 25 0.403 50 0.794 Unknown sample 0.201 1. Calculate the concentration of phosphorus prepared in the series of calibration curve. 2. Assuming a linear relationship between the variables, determine the linear least- squares equation and correlation coefficient. e

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A series of standard calibration solutions were prepared in 100 mL volumetric flask by reacting
standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid
complex to produce the characteristic blue color. The concentration of stock solution was 100 mg/L. The
absorbance, A were measured using colorimetric spectrometer as shown in the below table.
A calibration curve was plotted against the concentration of phosphorous.
Volume of Phosphorous
from stock solution (mL)
Concentration of
Absorbance
phosphorus (mg/L)
1
0.001
5
0.086
10
0.173
25
0.403
50
0.794
Unknown sample
0.201
1. Calculate the concentration of phosphorus prepared in the series of calibration
curve.
2. Assuming a linear relationship between the variables, determine the linear least-
squares equation and correlation coefficient.
Transcribed Image Text:A series of standard calibration solutions were prepared in 100 mL volumetric flask by reacting standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce the characteristic blue color. The concentration of stock solution was 100 mg/L. The absorbance, A were measured using colorimetric spectrometer as shown in the below table. A calibration curve was plotted against the concentration of phosphorous. Volume of Phosphorous from stock solution (mL) Concentration of Absorbance phosphorus (mg/L) 1 0.001 5 0.086 10 0.173 25 0.403 50 0.794 Unknown sample 0.201 1. Calculate the concentration of phosphorus prepared in the series of calibration curve. 2. Assuming a linear relationship between the variables, determine the linear least- squares equation and correlation coefficient.
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