A sample of a red dye and a sample of a blue dye both have the same concentration. Their Absorbance is measured using the same spectrophotometer. At a particular wavelength, will both samples give the same Absorbance reading? Why or why not?
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A sample of a red dye and a sample of a blue dye both have the same concentration. Their
Absorbance is measured using the same spectrophotometer. At a particular wavelength, will
both samples give the same Absorbance reading? Why or why not?
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- How will you use a solution of known concentration of one of the food dyes to determine the relationship between concentration and absorbance? Be sure to explain how you determined the wavelength for your analysis.This absorbance value (no wavelength where the maximum absorption occurs) corresponds to what % Transmittance value?4. Beer's Law states that absorbance of a compound is directly proportional to the log of the concentration of the absorbing species. * True False
- Assume that you have an "X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220 0.050 0.440 0.100 0.760 0.150 1.250 0.200 1,460 unknown 0,640The absorbance of a mixture at a specified wavelength is the total of the absorbances of each component at that wavelength. O True O FalseYou measure that the %T of a sample at 850 nm is 20%. What is the absorbance of this sample? Report your answer to three decimal places.
- a) A colorimeter is a type of spectrophotometer which is used to measures the light transmitted through a sample at a specific wavelength. True or False. b) As the cell path length shortens, the percent transmittance increases. True or false. c) As the absorbance value increase, the transmittance decrease. True or false. d) Ultraviolet-visible spectroscopy measures the absorption of plane-polarized light to determine the three-dimensional structure of a compound. True or false.Which of the following is the reason that a more concentrated dye solution absorbs more light than a less concentrated solution? There are fewer molecules of dye in solution that can absorb photons, meaning more photons reach the detector, increasing absorbance. There are more molecules of dye in solution that can absorb photons, meaning fewer photons reach the detector, increasing absorbance. There are more molecules of dye in solution that can absorb isotopes. There are more molecules of dye in solution that can absorb electrons. There are fewer molecules of dye in solution that can absorb electrons, meaning more electrons reach the detector, increasing absorbance.What is absorbance? Group of answer choices The amount of light not passing through a sample measured in units of M-1⋅cm-1. The amount of light passing through a sample measured in units of M-1. The amount of light passing through a sample measured in units of M-1⋅cm-1. The amount of light not passing through a sample measured in units of M-1. The amount of light not passing through a sample measured in units of J/s. The amount of light passing through a sample measured in units of J/s. The amount of light passing through a sample with dimensionless units. The amount of light not passing through a sample with dimensionless units.
- 2. Calculate the absorbance value (A) for a solution that absorbs 68.5% of the light striking it. (Hint: What is the percent transmittance?)Which is TRUE for spectroscopy of a mixture? A. The absorbance for a mixture at a particular wavelength is the sum of the absorbances for the components that absorb at the particular wavelength. B. Spectrophotometers can differentiate between mixture components that absorb at the same wavelength. C. Each component in a mixture has the same molar absorptivity at the same wavelength. D. The concentration for each component in a mixture is easily calculated by least squares for guesses of each component when the individual spectra are well resolved.A student generated the following calibration curve using Allura red. They measured the absorbance of a sample containing an unknown concentration of Allura red at the same wavelength. The absorbance of the unknown sample was equal to 0.5. Use the calibration curve to estimate the concentration of the dye in the sample.