A researcher wants to determine the concentration of beta-carotene in carrots. She obtains a 0.554 M stock solution of beta-carotene and creates Dilution A by transferring 23.00 mL of stock solution into a new container and diluting it with solvent to a final volume of 99.00 mL. She creates Dilution B by transferring 59.00 mL of stock solution into a new container and diluting it with solvent to a final volume of 73.00 mL. She creates Dilution C by transferring 8.00 mL of Dilution B into a new container and diluting it with solvent to a final volume of 25.00 mL. She measures the absorbance of each solution and obtains the values below. Plot the data in a spreadsheet software and determine the best-fit line. What is the slope of the best-fit line? Solution Absorbance Stock solution 0.971 Dilution A 0.273 Dilution B 0.597 Dilution C 0.294
A researcher wants to determine the concentration of beta-carotene in carrots. She obtains a 0.554 M stock solution of beta-carotene and creates Dilution A by transferring 23.00 mL of stock solution into a new container and diluting it with solvent to a final volume of 99.00 mL. She creates Dilution B by transferring 59.00 mL of stock solution into a new container and diluting it with solvent to a final volume of 73.00 mL. She creates Dilution C by transferring 8.00 mL of Dilution B into a new container and diluting it with solvent to a final volume of 25.00 mL. She measures the absorbance of each solution and obtains the values below. Plot the data in a spreadsheet software and determine the best-fit line. What is the slope of the best-fit line?
Solution | Absorbance |
Stock solution | 0.971 |
Dilution A | 0.273 |
Dilution B | 0.597 |
Dilution C | 0.294 |
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