I. A protein, X, was Isolated from a pathogenlc mlcroorganism. The proteln Is a vlrulencefactor whose path0genlclty lies In a heptapeptide of unknown sequence. After trypsincleavage of the heptapeptide from protein X, the peptlde's compOsition and sequencewas determined. The fOllowing were the results of the sequenclng process:1. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-aspand a mixture of amino acids were produced.2. When the same Intact peptide was treated with streptococcal protease, apentapeptide of composition asp, asN, cys, gly and ser and 2 amlno acidswere released.3. When the heptapeptlde was also treated with hydrOxylamine HCI, atripeptide and a tetrapeptide were obtained. The C-terminal amino acid ofthe tripeptide was asN.1) What is the sequence of the heptapeptide if it is composed of cys, asp, lys, asN, glyand ser only?2) What is the pl of the heptapeptide?

Proteins are formed of peptide bonds formed between the amine group and the carboxyl group of the…

Answered: A protein, X, was Isolated from a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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