Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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A polypeptide was treated with 2-mercarptoethanol and produced the following fragments:
Fragment 1:
STCYKEVCKRFC
Fragment 2:
ACWC
In another experiment, the same polypeptide (unreduced) was treated with thermolysin and
produced the following fragments: (Note: The amino acids in the parenthesis is NOT their
sequence.)
(W, C)
(K, E, Y)
(C, C, V, R, K, A)
(F, S, T, C, C)
Indicate the disulfide bonds in the polypeptide.
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Transcribed Image Text:A polypeptide was treated with 2-mercarptoethanol and produced the following fragments: Fragment 1: STCYKEVCKRFC Fragment 2: ACWC In another experiment, the same polypeptide (unreduced) was treated with thermolysin and produced the following fragments: (Note: The amino acids in the parenthesis is NOT their sequence.) (W, C) (K, E, Y) (C, C, V, R, K, A) (F, S, T, C, C) Indicate the disulfide bonds in the polypeptide.
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