A, nim 1.0- 0.8- O min 0.6- 0.5 5 10 0.4- 25 40 70 100 0.2- 120 0.0+ 200 350 400 Fig. 28 Time course es occurring during the UVB/UVA irradiation of octyl methoxycinnamate (octinoxate) under aerobic conditions in (a) aqueous media, and in (b) n-hexane. Repro- duced from Ref. [111] with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry 7. Using the figure from the paper above, why is it considered best practice to reapply sunscreen if you plan to be in the sun for long periods? absorbance

A, nim 1.0- 0.8- O min 0.6- 0.5 5 10 0.4- 25 40 70 100 0.2- 120 0.0+ 200 350 400 Fig. 28 Time course es occurring during the UVB/UVA irradiation of octyl methoxycinnamate (octinoxate) under aerobic conditions in (a) aqueous media, and in (b) n-hexane. Repro- duced from Ref. [111] with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry 7. Using the figure from the paper above, why is it considered best practice to reapply sunscreen if you plan to be in the sun for long periods? absorbance

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter31: Introduction To Analytical Separations

Section: Chapter Questions

Problem 31.26QAP

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A UVIVIS molecular absorption spectrometric determination of analyte X in aqueous solution gave the following calibration data (absorbances corrected for the blank). Estimate the concentration of Xin an aqueous sample that yielded an instrument response of 0.238 (A 1.00- mL cuvette was used for all absorbance measurement s) OA. 7.7 ppm OB. 8.0 ppm, 148 O c. 23.8 ppm O D.0.0308 ppb OE. 0.8 ppm Concentration (ppm X) 1.0 2.0 5.0 7.0 8.5 10.0 Absorbance 0.031 0.068 0.150 0.221 0.262 0.308 3:49 PM
You have measured the blank for a determination of arsenic in food samples by hydride-generation atomic fluorescence spectrometry. The blank values are: 0.23 ppb, 0.14 ppb, 0.16 ppb, 0.28 ppb, 0.18 ppb, 0.09 ppb, 0.10 ppb, 0.20 ppb, 0.15 ppb, 0.21 ppb As. What is the LOD?
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Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by binding Coomassie Brilliant Blue dye to protein standards and unknown, leading to a shift in the absorbance maximum of the dye. A stock solution of BSA is available (10 mg/ml) from which the protein standards were prepared. Bradford reagent, 290 ul, was pipetted into a transparent 96-well microplate. 10 ul of the protein dilution was added followed by mixing in the wells. After 5 min of incubation at room temperature, the plate was read in spectrum mode with the absorbance spectrometer of the microplate reader. The spectrum of the dye not bound to protein was also determined. Create a standard curve or regression equation that related the protein concentration and absorbance. An unknown BSA sample was prepared the same way as the standard and the OD was 0.35. determine the original concentration of the protein solution. The measurements can be seen below. 0.0625 Protein Concentration…
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