A mixture of three peptides is provided to you. The sequences of the three peptides are known: 1. HHHHH (pl = 7.16) 2. wwwww (pl = 5.52) 3. YYYYE (pl= 4.00) Which of these three peptides would not have absorbance at 280 nm? Select all that apply Peptide 1 Peptide 2 Peptide 3 Which of the following purification protocols would best separate the three peptides in question #4 without using denaturing conditions? Size exclusion and hydrophobic interaction chromatography O Anion exchange chromatography and hydrophobic interaction chromatography O Maltose-affinity chromatography and isoelectric focusing O SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and anion exchange chromatography

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