Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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A marine microorganism contains an enzyme that hydrolyzes glucose-6-sulfate. The activity test is based on the measurement of the rate of glucose formation. The enzyme in a cell-free extract has a KM of 6.7 x 10-9 M and a Vmax of 300 nM min-1 . Galactose-6-sulfate is a competitive inhibitor of the enzyme. To a concentration of glucose-6-sulfate 2 x 10-5 M and galactose-6-sulfate 10-5 M, the speed initial is 1.5 nM min-1 . Calculate the Ki for galactose-6-sulfate.
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