Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer ( 1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl₂ :1.2 mM (FW: 95.21) K₂HPO4 :2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.
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Transcribed Image Text:A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer ( 1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl₂ :1.2 mM (FW: 95.21) K₂HPO4 :2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.
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