help please (a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin methodwas used and the absorbance data in Table 1 was generated.Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sampleA.Tube Number Standard (mM)/ Test sample A00.0250.050.0750.1Test sample A (undiluted)e(1)(ii)12345678Test sample A (2-fold dilution)Test sample A (5-fold dilution)Absorbance @575nm00.1260.2310.3550.4790.7230.3570.143Using the data in Table 1 estimate the amino acid concentration of test sample A.Given the true value of test sample A is 0.15mM, comment on the accuracy andprecision of the experimental data.Describe the principle of the Ninhydrin method for quantitation of amino acids.

#(a): #(i): First, we have to draw the Absorbance versus Concentration(mM) plot of the standard…

(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 1 0 2 0.025 3 0.05 4 0.075 0.1 (i) (ii) 5 Absorbance @575nm 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimernal data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 1 0 2 0.025 3 0.05 4 0.075 0.1 (i) (ii) 5 Absorbance @575nm 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimernal data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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