(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 1 0 2 0.025 3 0.05 4 0.075 0.1 (i) (ii) 5 Absorbance @575nm 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimernal data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

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Please complete the calculations related to pH, pOH, [H3O+], and [OH-]. Remember there are special rules related to significant digits when working with pH, pOH, [H3O+], and [OH-].  A sample of household vinegar was measured to have a hydronium ion concentration of 5.8 x 10-3mol/L.  Determine the pH of the sample.  Hand soap is effective at preventing bacterial contamination.  Calculate the hydronium ion concentration in a brand of hand soap with a pH of 10.1Human saliva contains enzymes that start the process of digestion.  Human saliva typically has a hydroxide ion concentration of 1.2 x 10-8 mol/L.  Determine the pOH of saliva. Human blood is slightly basic.  Calculate the hydroxide ion concentration in a sample of blood with a pOH of 6.55

a) Determine the concentration of acetic acid in the 25.00 mL aliquot. b) Determine the concentration of acetic acid in the 10.0mL of vinegar. concentration NaOH = 0.101M Trial 3 Initial NaOH level 35.1 mL Final NaOH level 52.5mL The volume of NaOH used to reach the endpoint 17.4 mL

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