A biochemist is trying to determine the type of proteases they have isolated from walrus blubber.The three proteases and their relative activities in the presence of the indicated non-specificirreversible inhibitors are shown in the table below:Protease aProtease BProtease yGiven this data, please answer the following question:The catalytic site of Protease y contains an important:сHY+ lodoacetateNormal ActivityNormal ActivityNo ActivityR+ TetranitromethaneNormal ActivityNo ActivityNormal Activity

The modification of amino acid side chains using amino acid specific covalent labeling is an…

Answered: A biochemist is trying to determine the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1. Please fully explain (use illustrate where appropriate) the Modes of Enzyme Catalysis exemplified by the serine protease: Chymotrypsin. In your answer discuss employing the illustration whenever possible: the overall reaction mechanism, stability of the reaction transition state, proximity and orientation effects, acid-base catalysis, and covalent catalysis. (c) (0) Ap Asp Toe His Asp 10 C-N bond cleavage HN Ho Ser Ger Binding of substi 196 Ser Gly alto video LBHB NH Sere HAR Proton donation by H (h) Fel of amino product yest OHN Hig Ser Ap (0) Formation of covalent (ES) Alp Me complex Serios
Identify the type of enzyme inhibition each of the following inhibitor characteristics is associated with: 1. An inhibitor that decreases enzyme activity by binding to a site on the enzyme other that the active site. 2. An inhibitor that inactivates enzymes by forming a strong covalent bond of the enzyme acitve site.
give an example like the reaction mechanism of serin protease for example, with pictures & full explanation
Why is Methotrexate used in chemotherapy? How does it work in terms of enzyme inhibition that is involved in its mechanism action?
Triclosan is a broad-spectrum antibacterial agent used in many household products. It is structurally similar to polychlorobiphenylol, a substrate that binds to the active site of the enzyme sulfotransferase. Based on these facts, you can conclude that triclosan most likely: lowers the activation energy of chemical reactions. functions as an enzyme when sulfotransferase is absent. is a competitive inhibitor. acts as a noncompetitive inhibitor.
Give a complete and well descriptive definition of the following:1.1 Fractional saturation1.2Allostery1.3 Metal activated enzymes 1.4 Metalloenzymes1.5 Acid-base catalysis
The active site of an enzyme has the following amino acid residues as critical mediators of catalysis: Ser-134, His-347, Tyr-121 and Glu - 406. What are the most likely specific mechanisms of catalysis by these amino acid residues?
3
A schematic representation of the enzyme IspD complexed to inhibitor 3, and a series of inhibitors 3-5 are shown below. Ala202 lle240 mwww NH NH Val263 ОН www HN N- lle177 HN 'N' CI 3 X = N 4 X = C-CN 5 X = C-COO IC50 274 µM IC50 140 nM IC50 35 nM NH2 HN Val266 N -N O-H---- N HN %3D Arg157 HN wwww lle265 Explain why structure 4 is a more potent inhibitor (lower IC50 value) than inhibitor 3 and why structure 5 is a much weaker inhibitor (higher IC50 value) than 3 and 4.
Small molecules are used as inhibitors of protein action - as drugs. They most often do this by blocking the active site within the protein. Potential drugs can be screened computationally to determine if they are strongly bound to the protein. Figure 1 shows a possible conformation of a candidate drug molecule, 4-bromo-2- carboxymethylamide-pyrrole (abbreviation: BCMAP) at the active site of a protein (abbreviation: PR). Figure 2 shows the full protein structure whilst figure 3 shows a known inhibitor of the protein at the site, overlayed with another calculated conformer of BCMAP. (a) Explain what types of interactions, both intermolecular and intramolecular, that a molecular mechanics forcefield must be able to describe in order to be able to accurately determine the geometry of BCMAP in the protein. Identify which interactions will be the most important to describe accurately. Figure 1.4-bromo-2-carboxymethylamide-pyrrole (BCMAP) (C, N, O, and Br atoms in yellow, blue, red, and…
You are trying to confirm that an enzyme you are studying has the following amino acid in its active site, and that this amino is critical for catalysis: HN- H3NⓇ Which of the following inhibitors could you use to provide evidence of the importance of this amino acid for enzyme function? Tetranitromethane 2,3-Butanedione Diethylpyrocarbonate lodoacetate Nevirapine
what's the biggest mechanism difference between aspartate protease and serine protease?

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