A biochemist has 100 mL of a buffered solution at pH 7.5. The concentration of the buffer is 0.1 M, and the pKa of the buffer is 7.9. the biochemist adds 80. mL of a 0.1 N KOH solution to the above buffered solution, what will be the final pH to the nearest tenth of a unit?
Q: Peptide A: AFEQHSR Calculate the isoelectric point of Peptide A using the pKa values in Table 4-1 of…
A: A peptide is a short chain of amino acid residue linked via a peptide bond. Recall that: Amino acid…
Q: A mutation of Lysine 82 (in the DPG-binding pocket) to Arginine in beta sub-unit will most likely…
A: Changing the genetic code of a living thing permanently and inherited is known as a mutation. The…
Q: Would the base pair Guanosine and Cytosine be more or less stable at pH 10
A: The stability of a base pair like Guanosine (G) and Cytosine (C) can be influenced by pH, but in the…
Q: complete the sentence. In the Sanger method, the carbon atom of the reagent fluorodinitrobenzene…
A: The Sanger reagent (1-fluoro-2,4-dinitrobenzene) is used to determine the N-terminal amino acid in…
Q: For the reaction 2NH3(g) 3H₂(g) + N₂(g) the equilibrium concentrations were found to be [NH3] =…
A: The equilibrium constant of a chemical reaction is the ratio of the product of the active masses of…
Q: The amide hydrogen atoms of peptide bonds within proteins can exchange with protons in a solvent. In…
A: Hydrogen-deuterium exchange (HDX) is a technique that can be used to study the structure and…
Q: change for formation of an ES complex if the KD for this enzyme/ligand pair is 0.28µM? Give your…
A: The free energy change (ΔG) for the formation of an ES complex can be calculated using the…
Q: Decide whether each of the following statements is true about chitin deacetylase. It tremendously…
A: Chitin deacetylase is an enzyme found in a wide range of microorganisms, including fungi and…
Q: Proline is sometimes found as one of the first four residues of an alpha helix. Proline is less…
A: Proline is one of the 20 standard amino acids which has an amino group (NH2), a carboxyl group…
Q: The place on the enzyme where the substrate binds is called the: a) coenzyme b) transition state…
A: An enzyme is usually made up of protein with different amino acids linked together by peptide bonds.…
Q: DNA sometimes has chemical groups attached, called methyl groups, that affect gene expression.…
A: Methyl groups on DNA have a significant role in epigenetics and gene regulation and have a strong…
Q: 7. Draw a diagram of the heme complexes in deoxyhemoglobin and oxyhemoglobin (complete porphyrin…
A: A heme complex is a critical molecular structure found in various biological molecules, such as…
Q: Refer to the figure below, what are the following forces of attraction is involved in the protein…
A: The tertiary structure of the protein is a 3D structure established and stabilised by the…
Q: Beta barrel is a type of: a) subunit b)motif c) domain d) transport protein
A: Beta barrel is a protein structure that consists of beta sheets. A closed barrel or toroidal…
Q: 1. The peptide below was isolated from fermented milk and shown to have antioxidant properties…
A: Since you have posted a question with multiple sub parts, we will provide the solution only to the…
Q: What are the molecular weights of the protein X polypeptides? (pick 1 only) - 50 kDa each - 60 kDa…
A: Native PAGE is a technique that uses non-denaturing gels (in the absence of SDS or any denaturing…
Q: Complete the data missing from the table, which shows assay data from the purification protocol of a…
A: A protein purification assay will include multiple protein purification techniques, conducted one…
Q: Identify the applications for gel filtration chromatography. separation of components in a mixture…
A: Chromatography techniques are used to separate components of a mixture by the virtue of differences…
Q: A lysine is burined inside a protein surrounded by hydrophobic resiudes, how does this affect the…
A: Answer :- The behavior of amino acid residues within a protein is profoundly influenced by their…
Q: Draw a diagram of the hydrogen bonding interactions in a right-handed alpha helix (show all main…
A: There are four classes of biological macromolecules: proteins, nucleic acid, carbohydrates and…
Q: Which of the reactions are spontaneous (favorable)? L-malate + NAD+ oxaloacetate + NADH + H+ AG =…
A: Gibbs free energy change (ΔG) is a thermodynamic quantity that represents the maximum reversible…
Q: Which mechanism does NOT regulate the activity of an enzyme? a competitive inhibitor binding to the…
A: Enzyme inhibition is a technique used by our body to regulate the activity of enzymes. Enzyme…
Q: Carbonic anhydrase catalyzes the reaction between carbon dioxide and water to produce carbonic acid.…
A: The major components of enzyme catalyzed reactions are substrate(s), enzyme(s) and product(s). A…
Q: Gamma turns are divided into two classes based on the phi and psi rotational bond angles of the…
A: An illustration of the permitted and forbidden areas of the phi and psi dihedral angles in the…
Q: When electrons are removed from a food molecule,the moleculeis conducting anendergonic reaction.…
A: Endergonic reactions are reactions that require an input of energy (heat) to take place. Exergonic…
Q: 1: QAMGRAGDLKYLGLHSV Peptide 2: ALMALFMVMALVLVSVLFIA Peptide 3: MVEDLLKQIARYLISE (a) Circle all of…
A: We are authorized to provide three sub parts at a time; since you have not mentioned which part, we…
Q: Many enzyme mechanisms involve transferring protons. Sidechains of an enzyme can act as catalytic…
A: Described in the image is the reaction catalysed by triosephosphate isomerase. In an electron…
Q: Which of the following biomolecules is not present in the cell membrane (in any form Proteins O…
A: Biological membranes are bilayered structures that surround the cell or organelle and act as…
Q: Which nucleotide forms are used in the cellular reactions for DNA replication? a. 3' triphophates b.…
A: DNA replication is the process by which a new DNA molecule is synthesized using an already existing…
Q: For this oligonucleotide, • Classify if RNA or DNA? Justify your choice. • Determine the sequence…
A: An oligonucleotide is a short sequence of nucleotides that is often made up of bases from DNA or…
Q: In the lab where you work, a graduate student has created a K+ leak channel that is mutated in such…
A: Membrane potential is the difference in electrical charge between the inside and the outside of a…
Q: 19. The bond between a Carboxyl and an Amino group is what? hydrogen bonds peptide linkages a. b. C.…
A: Proteins are high molecular weight, three-dimensional biochemical polymers that serve diverse…
Q: For each amino acid, show the structure, 1-letter abbreviation, 3-letter abbreviation, class…
A: Amino acids are organic compounds that contain both amino and carboxylic acid groups. Amino acids…
Q: An intermediate folding stage seen in protein denaturation or renaturation is called : a) domain b)…
A: An intermediate folding stage seen in protein denaturation or renaturation is called :and option :a)…
Q: The reaction coordinate diagram shown below is for the mitochondrial enzyme ATP synthase. G KJ/mol…
A: A reaction coordination graph is a graphical representation of the free energy changes that occur…
Q: For the structures below, please indicate whether the compound is reducing or non-reducing, and…
A: Carbohydrates that have a free anomeric hydroxyl group can reduce cupric ions to cuprous ions. Such…
Q: In your Amphipathic Home Lab, describe ach of these statements as true or false: Oil and water form…
A: Amphipathic compounds are molecules with both water-attracting (hydrophilic) and water-repelling…
Q: Consider an alpha-helix comprised of twelve amino acid residues. How many hydrogen bonds should be…
A: Proteins are linear polymers of amino acids which form the basic unit. Amino acids consist of a…
Q: Determine the relationship between compounds 1 and 2 Is it A - meso compounds b - enantiomers C-…
A: Stereoisomers are the isomers in which molecular formula are same for both the isomers but they…
Q: Which statement about the glycoprotein shown below is true? OH CH₂OH OH HN HN-C O CH3 H₂ Protein CH…
A: Chemically, carbohydrates are polyhydroxy aldehydes or ketones. Monosaccharides are single unit…
Q: It has been estimated that for the and P = 70,000 in 1976. Model this population. Then, revise the…
A: Since you have posted a question with multiple sub parts, we will provide the solution only to the…
Q: Draw the amino acids serine and threomine bonded together with a petide bond.
A: Amino acid
Q: 47. This is NOT true in regards to the functional group of monosaccharides A. It is the carbon…
A: In the realm of biochemistry and carbohydrate chemistry, the properties and behaviors of…
Q: 13. What is the main function of the carnitine shuttle system? a. To aid in the assembly of…
A: Carnitine shuttle system is a special mechanism that consists of two transport proteins called…
Q: Consider the reaction. 2XY+Z When the concentration of X is doubled, the reaction rate increases by…
A:
Q: A lysine is on the surface of a protein, near an aspartate. How does the aspartate alter the pKa of…
A: The pKa of an amino acid's side chain determines whether it is protonated (+) or deprotonated (-)…
Q: Give the Steps, Enzyme/s involved, Electron carriers, ATP Generation, End product and significance…
A: Aerobic respiration is a collection of three metabolic pathways that generate ATP by the oxidation…
Q: If the half-life for the decomposition of a drug is 4 hours, how long will it take for 225 mg of the…
A: In order to solve this problem, first we need to calculate the decay constant (). The half life…
Q: In the absence of an enzyme, a reaction has a Keq = 10¹5. i. Is AGO for the reaction + or -? ii.…
A: In a general reaction such as:aA + bB ⇌ cC + dDAt equilibrium (steady state), the concentration of…
Q: The ESI-MS spectrum in positive ionization mode for lysozyme is obtained. a. What is the molecular…
A: The y-axis of the ESI-MS spectrum reflects relative abundance and the x-axis the m/z (mass charge)…
Step by step
Solved in 4 steps
- 50X TBE is 87 mM Tris-borate and 4 mM EDTA at pH 8.3. What is the molarity of each of the chemicals in 1X TBE buffer?You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.A 25.00 mL sample of 0.320 M LIOH is titrated with 0.750 M HNO3 at 25 °C. Calculate the initial pH before any titrant is added. pH = Calculate the pH of the solution after 5.00 mL of the titrant is added. pH =
- If 10g of salt was mixed in 1L water. What percent solution will this be.A biochemist has 100 mL of a 0.10 M solution of a weak acid with a pKa of 6.3. She adds 6.0 mL of 1.0 M HCl, which changes the pH to 5.7. What was the pH of the original solution?A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units units
- An aqueous solution of 0.2100 g of a mixture containing potassium cyanide and potassium chloride required 14.56 mL of 0.1000 M silver nitrate to produce a faint permanent turbidity; 30.00 mL more of the silver nitrate was added (an excess) and the precipitate of AgCl and AgCN filtered off from the solution. The filtrate and washings were titrated with 13.06 mL 0.1000 M thiocyanate solution. Calculate the percentages of KCl and KCN in the sample. Note: the appearance of faint turbidity indicates the formation of Ag(CN)2-An aqueous antifreeze solution is 45.0% ethylene glycol () by mass. The density of the solution is 1.05. Calculate the molality, molarity, and mole fraction of the ethylene glycolWhat is the molarity of a 30% solution of NaCI
- A beaker with 1.40x102 mL of an acetic acid buffer with a pH of 5.000 is sitting on a benchtop. The total molarity of acid and conjugate base in this buffer is 0.100 M. A student adds 7.00 mL of a 0.490 M HCl solution to the beaker. How much will the pH change? The pKa of acetic acid is 4.740. Express your answer numerically to two decimal places. Use a minus ( - ) sign if the pH has decreased. View Available Hint(s) ΑΣφ ApH =You need a buff er at pH 7.5 for use in purifying a protein at 4°C. You have chosen Tris, pK 8.08, ΔH° = 50 kJ · mol−1. You carefully make up 0.01 M Tris buffer, pH 7.5 at 25°C, and store it in the cold to equilibrate it to the temperature of the purifi cation. When you measure the pH of the temperature-equilibrated buff er it has increased to 8.1. What is the explanation for this increase? How can you avoid this problem?A buffer solution is composed of 1.00 mol of acid and 1.75 mol of the conjugate base. If the pKa of the acid is 3.60, what is the pH of the buffer? pH=