A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01023 M I3 – to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3 – was back-titrated with 13.82 mL of 0.07203 M Na2 S2O3 to starch indicator end point. What is the concentration of ascorbic acid in the orange juice? Report the concentration ascorbic acid in mg/100 mL.  C6H8O6 + I3 – → C6H6O6 + 3I - + 2H+  I3 – + 2S2O3 2- → S4O6 2- + 3I2  Answer: 48.5  mg/100 mL

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A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01023 M I3 – to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3 – was back-titrated with 13.82 mL of 0.07203 M Na2 S2O3 to starch indicator end point. What is the concentration of ascorbic acid in the orange juice? Report the concentration ascorbic acid in mg/100 mL.

 C6H8O6 + I3 – → C6H6O6 + 3I - + 2H+

 I3 – + 2S2O3 2- → S4O6 2- + 3I2 

Answer: 48.5  mg/100 mL

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