8. Yeast genes have cis-acting elements upstream oftheir promoters, similar to enhancers, called upstreamactivating sequences or UASs. Several target genes involved in galactose utilization are regulated by onetype of UAS called UASG, which has four bindingsites for an activator called GAL4. Two target genesregulated by UASG are GAL7 and GAL10. The GAL80 protein is an indirect repressor of GAL7 and GAL10transcription: At UASG, GAL80 binds to GAL4 protein and blocks GAL4’s activation domain. In thepresence of galactose, GAL80 no longer binds GAL4.In which gene(s) (GAL4 and/or GAL80) shouldyou be able to isolate mutations that allow the constitutive expression of the target genes GAL7 andGAL10 in the absence of galactose? In each case,what characteristics of the protein would the mutationdisrupt?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
8. Yeast genes have cis-acting elements upstream of
their promoters, similar to enhancers, called upstream
activating sequences or UASs. Several target genes involved in galactose utilization are regulated by one
type of UAS called UASG, which has four binding
sites for an activator called GAL4. Two target genes
regulated by UASG are GAL7 and GAL10. The GAL80 protein is an indirect repressor of GAL7 and GAL10
transcription: At UASG, GAL80 binds to GAL4 protein and blocks GAL4’s activation domain. In the
presence of galactose, GAL80 no longer binds GAL4.
In which gene(s) (GAL4 and/or GAL80) should
you be able to isolate mutations that allow the constitutive expression of the target genes GAL7 and
GAL10 in the absence of galactose? In each case,
what characteristics of the protein would the mutation
disrupt?
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