5. In the tomato the mutant genes o (oblate flattened fruit), p (peach = hairy fruit), and s (compound inflorescence = many flowers in a cluster) were found to be linked on chromosome 2. A testcross with a heterozygote was done, yielding the following progeny:. || || || || +++ = 73 ++s = 348 +p+ = +ps = o++ = 110 o+s = op+ ops = va 382 = 609 96 2 306 63 Determine a) the sequence and arrangement of the genes in the heterozygous parent; and b) the distance between the three genes.
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- In tomato, the following genes are located on chromosome 2: + tall plant d dwarf plant + uniformly green leaves m mottled green leaves + smooth fruit p pubescent (hairy) fruit Results of the cross +++ / dmp and dmp / dmp were: + + + = 470 + m p = 1 + + p = 14 d + p = 25 d + + = 0 d m p = 441 + m + = 19 d m + = 30 Identify the single and double crossovers among the progeny.7-5. In corn a pair of genes determines leaf shape and another pair determines pollen shape. A ragged-leafed plant with round pollen was crossed to a ragged-leafed plant with angular-pollen, and the resultant progeny were classified as follows: Class Phenotypes 186 ragged-leaf round-pollen 11 IV 174 ragged-leaf angular-pollen 57 smooth-leaf round-pollen Total 63/480 smooth-leaf angular-pollen (a) Using alphabetical letters of your choice, designate the genes for the different leaf and pollen characters. (b) On the basis of the symbols given in (a) 1 provide genotypes two parents. (c) (forth)/(nbers) According to your hypothesis what would you have expected for each of the four classes of progeny? (d) After re viewing Chapter 8, use the chi-square method (p. 132) to test your hypothesis and indicate whether you accept or reject it.2. Drosophila females heterozygous for three recessive x-linked markers y (yellow bodies), ct (cut wings), and m (miniature wings) were crossed to y ct m males and the following progeny were obtained. F1 Phenotype Yellow, cut, miniature Wildtype Yellow Cut, miniature miniature Number cut 60 63 20 13 Yellow, cut 15 Yellow, miniature 5 22 2 a) F1 Genotype b) Recombinant or Parental? c) SCO, DCO, or NONE? a) Fill in the table with the genotypes of each progeny type, use + to indicate wildtype/dominant traits, show separate homologues. b) In the table, identify whether each progeny type is Recombinant (R) or Parental (P). c) In the table, identify whether each progeny type results from a single crossover (SCO), a double crossover (DCO), or no crossing over (NONE). d) Diagram the parental cross, clearly indicating allelic contributions from each homologue of parental flies. Heterozygous wild-type Female Fly X Hemizygous mutant Male fly // // e) Draw the genetic map showing correct order and…
- n corn, male sterility is controlled by maternal cytoplasmic elements. This phenotype renders the male part of the corn plants (i.e the tassel) unable to produce fertile pollen; the female parts, however, remain receptive to pollination by pollen from male fertile corn plants. However, the presence of a nuclear fertility restorer gene F restores fertility to male sterile lines sing the cardboard chips, simulate the crosses indicated below. Give the genotypes and phenotypes of the offsprings in each cross, and properly label the nucleus and the cytoplasm of each individual in the cross Legend male sterile cytoplasm Male fertile cytoplasm FF nucleus Ff nucleus ff nucleus A. Male sterile female x FF male Explain the phenotype of the offspring B. Male sterile female x Ff male Explain the phenotype of the offspringn corn, male sterility is controlled by maternal cytoplasmic elements. This phenotype renders the male part of the corn plants (i.e the tassel) unable to produce fertile pollen; the female parts, however, remain receptive to pollination by pollen from male fertile corn plants. However, the presence of a nuclear fertility restorer gene F restores fertility to male sterile lines Using the cardboard chips, simulate the crosses indicated below. Give the genotypes and phenotypes of the offsprings in each cross, and properly label the nucleus and the cytoplasm each individual in the cross Legend male sterile cytoplasm Male fertile cytoplasm FF nucleus Ff nucleus ff nucleus A. Male sterile female x FF male Explain the phenotype of the offspring B. Male sterile female x Ff male Explain the phenotype of the offspring3. In the test cross r/+ s/+ w/+ z/+ rswz/rswz, the following offspring are obtained: X Phenotype r st w+ z r stw z Number 359 47 4 rs w z+ rs w+z+ 98 rt st w z 92 r+ s w z+ 351 r+ st w+ z 6 r+s w+ z+ a) Construct a map of the genes showing their relative distance apart. 43 b) Is there any interference in this region? Note: the listed gene order is arbitrary. Can you deduce the proper order by inspection?
- In a haploid fungus, the genes al-2 and arg-6 are 30 m.u.apart on chromosome 1, and the genes lys-5 and met-1are 20 m.u. apart on chromosome 6. In a crossal-2 + ; + met-1 × + arg-6 ; lys-5 +what proportion of progeny would be prototrophic + + ;+ +?1. The allele G for yellow stigma is completely dominant to green (g). Supposingtwo strains of autotetraploid plants are available and their genotypes are as follows:GGgg – in this plant the gene is close to the centromereGggg – in this plant the gene is far from the centromere If these two plants are crossed:a) provide the gametes that can be obtained from the two plants;b) provide the genotypic and phenotypic ratios of the offspring. 2. Consider the illustration below. Diagram the configuration you would observe at Anaphase I if crossing-over happens within the inversion. (IMAGE ATTACHED)In corn, the following genes are linked on chromosome 3: va - variable sterile v - virescent gl - glossy leaves Two homozygous plants were crossed and produced an all-normal F1. The F1 was test-crossed, progeny phenotypes appeared as follows: 235 virescent 40 variable sterile 48 virescent, glossy7 glossy 270 variable sterile, glossy 4 variable sterile, virescent60 wild 62 variable sterile, virescent, glossy Show the diagram of the chromosome map for the 3 loci in this format: a--7.00 cM--b--8.00 cM--c--9.00 cM What is the correct sequence of the three gene loci? What is the coefficient of coincidence (COC) and the interference in this cross?
- 6. In Drosophila melanogaster, the wild-type eye colour is dark red. A pure-breeding mutant strain with white eyes also exists. When reciprocal crosses are made between (i) a white-eyed male a wild-type female, and (ii) a white-eyed female a wild-type male, the progeny are as follows: (1) P: White-eyed male x red-eyed female F1: red-eyed males and females F2: 100 progeny; 27 red-eved males, 24 white-eyed males, 49 red-eyed females (ii) P: red eyed male x white-eyed female F1: white-eyed males and red-eyed females F2: 100 progeny; 23 red-eved males 26 white-eyed males, 28 red-eyed females, 23 white-eyed females. How do you interpret these data?In corn, the genes v (virescent seedlings), pr (red aleurone), and bm (brown midrib) are all on chromosome 5, but not necessarily in the order given. The cross: v+ pr bm/ v pr+ bm+ with v pr bm/ v pr bm produces 1000 progeny with the following phenotypes: v+ pr bm 226 v pr+ bm+ 229 v+ pr bm+ 153 v pr+ bm 185 v+ pr+ bm 59 v pr bm+ 71 v+ pr+ bm+ 36 v pr bm 41 What is the gene order, and the (b) genetic map of these three genes?1. In a cross between mice the genotypes AB/ab x ab/ab, what is the recombination frequency if the progeny numbers are 72 AB/ab, 68 ab/ab, 17 Ab/ab, and 21 aB/ab? The alleles are shown for each chromosome, separated by a slash (/). . 2. A cross between fruit files with genotypes Aa Bb × aa bb produces the following progeny: 10 Aa Bb 40 Aa bb 40 aa Bb 10 aa bb Were the parental alleles in the coupling or repulsion configuration? Briefly explain. 3. Briefly describe how twin studies can reveal whether or not the appearance of a trait is strongly influenced by genetics.