5. Draw the reaction curve for the follow a) A reaction with an activation energy (EA) = 50 and free energy ( G) = 20

37. Consider the following energy diagram for an enzyme-catalyzed reaction. D B A C E G Which step is probably the slowest? a. A →B →C b. C D E c. E F G d. G H I

1. Answer the following questions, based on this reaction: S+E P ES EP2 E + P | B)Where is the transition state located?

2. The Km of a particular enzyme is 2.0 x 105 M. If the initial velocity (Vo) is 0.16 mmol/min for [S] = 0.15 M, what will be the initial velocity when [S] =2.0 x 10+M?

6. An enzyme catalyzed reaction has a KM of 1 mM and a Vmax of 5 nM/s. What is the reaction velocity when the substrate concentration is (a) 0.25 mM (b) 1.5 mM, and (c) 10 mM?

The following questions are based on the reaction A+ B ↔ C+D shown in Figure 8.1.   1. Which of the following terms best describes the progress of the reaction with respect to free energy change? a) endergonic, ∆G> 0 b) exergonic, ∆G> 0 c) exergonic, ∆G< 0 d) endergonic, ∆G< 0   2. Which of the following in Figure 8.1 remains unchanged by having an enzyme included? a) b b) d c) a       d) c   3. The part labeled “C” on the above graph represents a) Energy of activation without enzyme b) Energy of activation with enzyme c) Amount of free energy released d) amount of energy required for the reaction progress

12. Explain why those biological reactions that have their equilibria shifted towards theproducts have negative values for ΔGo of reactions. Explain how equilibria relates toGibbs free energy.