5 years old child came to emergency complaining of headache and fever (39.4°C). On examination, he has Confusion, difficulty in wake, Neck stiffness. sample sent to lab for further investigations. Gram stain show Gram-positive, spherical bacteria, alpha-hemolytic • What is the infection?

Definition Definition Technique that uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.