4. The graph below shows the titration curve that results when 50. mL of 0.50 M tromethamine is titrated with 0.100 M HC1. 12 A 10 B C 8 D 6 4 E 2 F -2 50 100 150 200 250 300 350 Volof HCI a. Label each of the parts on the graph including, buffer zone, optimum buffer zone, equivalence point, and equivalence point. 12 4 b. Determine the Kp for tromethamine using graph above. You must justify your answer by showing all of your work.

True or False: An IgM monomer can bind as many as 10 epitopes?

The order is for Streptomycin 1gm IM. You have a 5gm vial of Streptomycin . The label states to add 9ml of sterile water to yield 400mq / m * l How many ml will you give?

refer to the picture explain in detail the type of centrifugation which you will use to seperate imunoglobulin M and immunoglobulin G

This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?

Tryptophan binds TrpR. True False

¹C2²2S + E Sk3,0 where S is a T-cell of a particular variety, E is the corresponding (free, i.e. unbound) antigen, and C is a complex consisting of a T-cell bound to an antigen. Ø indicates the loss of T-cells due to decay as described above. 1. Let s, c and e be the concentration of S, C and E, respectively, in the body at time t. Assume that the background exposure to the disease results in a constant concentration eo of the antigen (free or bound) in the body. (b) (c) Consider the T-cell system. Assuming mass action kinetics, write down a system of ODEs for Show that ds dt S + E and k-1 where KM dc dt Show (include your working) that the 2D system of ODEs has two equilibria: (s*,c*) = (0,0) == : -k₁(eo — c)s + k_1c +2k₂c - k3s k-1+k2 k₁ (s*, c*) = ( k₂e0 k3 and KM, eo k2 dt' dt and KM = k₁(eo - c)s - k_1c - k₂c.