3. (a) What are the different methods of cell lysis? Discuss in detail.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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3. (a)
(b)
What are the different methods of cell lysis? Discuss in detail.
In Streptomyces filtration from an erythromycin broth using a
test filter, we find the following data:
Filtration time, sec
Volume of filtrate, L
5
10
20
30
0.040
0.055 0.080 0.095
The filter leaf has a total area of 0.009m² and the filtrate has a
viscosity of 1.1 cP. The pressure drop is 0.51m of mercury and the
feed contains 0.015 kg dry cake per liter. Determine the specific
cake resistance a and the medium resistance RM.
Transcribed Image Text:3. (a) (b) What are the different methods of cell lysis? Discuss in detail. In Streptomyces filtration from an erythromycin broth using a test filter, we find the following data: Filtration time, sec Volume of filtrate, L 5 10 20 30 0.040 0.055 0.080 0.095 The filter leaf has a total area of 0.009m² and the filtrate has a viscosity of 1.1 cP. The pressure drop is 0.51m of mercury and the feed contains 0.015 kg dry cake per liter. Determine the specific cake resistance a and the medium resistance RM.
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