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- Define the following terms: b. Isosmotic solutions c. Intravenous fluid Infiltration 2.What precautionary measures should be observed when isotonic solutions are used? 3. What precautionary measures should be observed when hypotonic solutions are used? 4. What are the Two types of feedback loops? Illustrate and explain briefly. 5. Complete the table below1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 9. 2. You are given the restriction enzyme Trll with the recognition sequence 5'...TGA^TCA... 3' to cut DNA. a) Would this cut result in blunt or sticky ends? Explain your answer. b) Restriction Enzyme Pmel contains the recognition sequence 5' ...GTTT^AAAC... 3'. Which restriction enzyme would result in more cuts, Trll or Pmel? Explain your answer. 3. 1. You are doing a DNA extraction using a Wizard kit as the first step to prove Joe Goldberg is guilty of murdering either his girlfriend, her best friend, her brother or her neighbour. Your tube contains a pellet and a supernatant after adding cold 70% ethanol and…Choose the best combination of depyrogenation methods: 0.45 um filtration + 0.22 µm filtration OUV+0.45 um filtration 0.22 µm filtration + UV treatment 0.45 um filtration + distillation 0.22 µm filtration + distillation
- Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What was the patient's creatinine clearance in mL/min? (Creatinine clearance = excretion rate of creatinine/plasma concentration creatinine). Show your work.Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What is the patient's approximate GFR (in mL/min) 2. Is this a normal GFR (in units of mL/min)? Did the patient sustain kidney damage?Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What is the patient's approximate GFR (in mL/min)?
- Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 3. How many mg of creatinine were in the 1.5 L urine specimen? How much creatinine appeared in the urine per hour? Show your work.You are given a cell stock A. You make a 1:100 dilution and load 10uL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T 1. What is the cell concentration of this diluted sample? 2. What is the cell concentration of original cell stock ? 3. If the original stock A has a total volume of 5mL, what is total number of cells in the A? 4. You decide to do a 1:1000 dilution to verify your counting. After loading and counting, you find out that you have 20 cells in average in each square. Do you think the new concentration is consistent with your previous result? If not, list all the possible errors that can cause this inconsistency. MacBook AirDILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?
- Using the data on enhanced coagulation and TTHM and HAA5 yield data and propose modification to the design necessary if your treatment process cannot meet the new regulation that specifies a maximum 80 and 60 ppb of TTHM and HAA5, respectively Table 2: DOC concentration achieved after enhanced coagulation pH Dose 5 6 7 8 0 5 5 5 5 10 3.07 3.21 3.42 3.59 20 2.05 2.63 3.17 3.37 40 1.71 2.22 3.00 3.32Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flow3) In hemodialysis the flow of blood and dialysate can operate concurrently or countei currently. What are the differences between these models. To remove the largest amount of solute ,which dialyzer would be preffered ? WHY?