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2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for Bamtl and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation.

2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for Bamtl and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Section B: Circular DNA Aatll 2641 Sspl 2525 Eco01091 2698 Pfol 46 BstAPI 179 Ndel 183 Ehel 235 Pdml 2318 206 MCS lacz \2510 146/ Bcgl 2239 Scal 2201. 236 476 493- PUC57 2710 bp BsaXI 683 Sapl 707 876. AflI, Pscl 830 Gsul 1808 Cfr101 1803 1659 1490 Eco311 1790 Eam11051 1718 rep (PMB1) \BseYl 1134 \Cail 1241 s TAA AAC AC00C CA TGA ATT CGA GCT COG TAC CTC GCG AAT GCA TCT AGA TAT COC ATC Cce fenen cCC TCG ACT OCA GAG GCC TạC ATG CAA GCT Tos r CGA GcC ATO GAG coC TTA COT AGA TCT ATA GCC TAG 0oc Coa osC AOC TGA COT CTC c00 ACG TACOTT CGA Ace lad W e A le A S Se P I e Ce S e P y g l An on cut A CAor CA Ator eo GCA TEA GA CCAATOACA AAGGAC S Thee e 1. You have a circular plasmid (PUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes?. Show all calculations. a. Pdml & Gsul b. Ndel, Sapl & BseYI c. Scal, Sspl & Ehel 2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for BamHỊ and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation. II bla (Ap)
Transcribed Image Text:Section B: Circular DNA Aatll 2641 Sspl 2525 Eco01091 2698 Pfol 46 BstAPI 179 Ndel 183 Ehel 235 Pdml 2318 206 MCS lacz \2510 146/ Bcgl 2239 Scal 2201. 236 476 493- PUC57 2710 bp BsaXI 683 Sapl 707 876. AflI, Pscl 830 Gsul 1808 Cfr101 1803 1659 1490 Eco311 1790 Eam11051 1718 rep (PMB1) \BseYl 1134 \Cail 1241 s TAA AAC AC00C CA TGA ATT CGA GCT COG TAC CTC GCG AAT GCA TCT AGA TAT COC ATC Cce fenen cCC TCG ACT OCA GAG GCC TạC ATG CAA GCT Tos r CGA GcC ATO GAG coC TTA COT AGA TCT ATA GCC TAG 0oc Coa osC AOC TGA COT CTC c00 ACG TACOTT CGA Ace lad W e A le A S Se P I e Ce S e P y g l An on cut A CAor CA Ator eo GCA TEA GA CCAATOACA AAGGAC S Thee e 1. You have a circular plasmid (PUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes?. Show all calculations. a. Pdml & Gsul b. Ndel, Sapl & BseYI c. Scal, Sspl & Ehel 2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for BamHỊ and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation. II bla (Ap)
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