2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for Bamtl and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation.
2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone has enzyme restriction sites for Bamtl and EcoRI, After investigation you see that the PUC57 plasmid also has these enzyme restriction sites in its multiple cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme digestion your amplicon that you wish to insert is 914 bp long. a. What will be the length of the recombinant plasmid after you have cloned your amplicon? Show your calculation(s). b. In the amplicon insert you have an enzyme restriction site for Ndel at 864 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be? Show your calculation.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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