2. The following DNA sequence was isolated from an interesting thermophilic bacterium. The Shine Dalgarno sequence used by this bacterium is AGGAGGU and is located approximately 5 nucleotides away (upstream) of the start codon of the ORF. 5' GGTCGTGCTGGGTTGAGAAATGCCTACGTGCTGCTAGTTGGGAGCTCCATAGGGGACCTCCTGGGT 3 A) What is the sequence of the ORF encoded on the resulting mRNA (include only the region from start to stop codon). Include 5' and 3' ends. What is the amino acid sequence of the protein generated from this ORF? (Include amino (N) and carboxy (C) termini express using three letter codes). If the bolded and underlined "G" is mutated into an "A", what effect would you expect this to have on the resu function of the protein? Explain briefly. CD
2. The following DNA sequence was isolated from an interesting thermophilic bacterium. The Shine Dalgarno sequence used by this bacterium is AGGAGGU and is located approximately 5 nucleotides away (upstream) of the start codon of the ORF. 5' GGTCGTGCTGGGTTGAGAAATGCCTACGTGCTGCTAGTTGGGAGCTCCATAGGGGACCTCCTGGGT 3 A) What is the sequence of the ORF encoded on the resulting mRNA (include only the region from start to stop codon). Include 5' and 3' ends. What is the amino acid sequence of the protein generated from this ORF? (Include amino (N) and carboxy (C) termini express using three letter codes). If the bolded and underlined "G" is mutated into an "A", what effect would you expect this to have on the resu function of the protein? Explain briefly. CD
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:2. The following DNA sequence was isolated from an interesting thermophilic bacterium. The Shine Dalgarno sequence
used by this bacterium is AGGAGGU and is located approximately 5 nucleotides away (upstream) of the start codon of
the ORF.
5' GGTCGTGCTGGGTTGAGAAATGCCTACGTGCTGCTAGTTGGGAGCTCCATAGGGGACCTCCTGGGT 3
A) What is the sequence of the ORF encoded on the resulting mRNA (include only the region from start to stop codon).
Include 5' and 3' ends.
What is the amino acid sequence of the protein generated from this ORF? (Include amino (N) and carboxy (C) termini
express using three letter codes).
If the bolded and underlined "G" is mutated into an "A", what effect would you expect this to have on the resu
function of the protein? Explain briefly.
CD
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