Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Read the article in the link and do a data analysis. What are the results and findings of the study? https://mdpi-res.com/d_attachment/ijms/ijms-21-05129/article_deploy/ijms-21-05129.pdf?version=1595262192
Notas 1:48 p. m. Vie 29 sep.
2. Results
2.1. Flow Cytometry with Canine CD Markers
Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of
surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical
for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3
expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was
low (3.0 ± 1.7%).
Int. J. Mol. Sci. 2020, 21, 5129
A
mdpi-res.com
CD90 APC-A
B
10
10
CD29 PE-A
C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21
CD90+
IgG
MSC canine CD29/45/90-BM02/CD29/45/9
CD29+
IgG
ALA
CD45 FITC-A
IgG
CD45+
3 of 21
Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note
confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses
of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+
(77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.
2.2. Multilineage Potential
Three-lineage potential was confirmed using the commercial StemPro® Differentiation Kit and
corresponding protocol for induction towards chondrogenic, osteogenic and adipogenic lineages.
After 14 days of incubation in cell-type corresponding differentiation medium, we confirmed that
canine BMMSC show a high degree of biomineralizing osteogenesis with visual staining of calcium
deposits expressing Alizarin Red positivity (Figure 2B). Furthermore, chondrocytes migrating from
spherical chondrocyte-like aggregates revealed intense Alcian Blue staining of glycoproteoglycans
(Figure 2D). In contrast to the high osteogenic and chondrogenic differentiation of canine BMMSC,
Commation and Oil Rai staini
me found a low de
with limite
38 %
expand button
Transcribed Image Text:Notas 1:48 p. m. Vie 29 sep. 2. Results 2.1. Flow Cytometry with Canine CD Markers Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3 expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was low (3.0 ± 1.7%). Int. J. Mol. Sci. 2020, 21, 5129 A mdpi-res.com CD90 APC-A B 10 10 CD29 PE-A C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21 CD90+ IgG MSC canine CD29/45/90-BM02/CD29/45/9 CD29+ IgG ALA CD45 FITC-A IgG CD45+ 3 of 21 Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+ (77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm. 2.2. Multilineage Potential Three-lineage potential was confirmed using the commercial StemPro® Differentiation Kit and corresponding protocol for induction towards chondrogenic, osteogenic and adipogenic lineages. After 14 days of incubation in cell-type corresponding differentiation medium, we confirmed that canine BMMSC show a high degree of biomineralizing osteogenesis with visual staining of calcium deposits expressing Alizarin Red positivity (Figure 2B). Furthermore, chondrocytes migrating from spherical chondrocyte-like aggregates revealed intense Alcian Blue staining of glycoproteoglycans (Figure 2D). In contrast to the high osteogenic and chondrogenic differentiation of canine BMMSC, Commation and Oil Rai staini me found a low de with limite 38 %
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