Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains
biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only
three buffer systems are available in their laboratory:
Buffer System Weak Acid Component
Ka
Conjugate Base Salt
PAH
Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO
SAD
Boric acid, H3BO3
5.81 x 107 NaH₂BO3
DOH
Hydrogen cyanide, HCN 6.20 X 10-10 NaCN
A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme?
In your solution sheet, show calculations and briefly explain to support your answer.
B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet
C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to
prepare 250 mL of a 0.650 M buffer pH 10.00.
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Transcribed Image Text:2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO SAD Boric acid, H3BO3 5.81 x 107 NaH₂BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
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