2. A chromatographic column is eluted with a solution containing a mixture of two components, X and Y. The chemical affinity of X for the stationary phase is less than that of Y and the chemical affinity for X for the mobile is greater than that of Y. Which substance will be eluted first? Explain your answer.

A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure.  Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places)  What other dye/s are present in dye C and what is the dye with least number of pigments?

Chromatography 1. External Standard Method A. Calculate the regression equation and the R-squared value. B. Calculate the concentration of cocaine found in the blood samples of the presidentiables. Which presidential candidate “reliably” tested positive for cocaine?   2. Internal Standard Method >Calculate the concentration of party drug X in the blood samples of the presidential candidates. >Suppose the LOD for party drug X is 0.200 ppm, which of the presidential candidates “reliably” tested for party drug X?