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1. What is the reason for inverting bottom part of the plate during point inoculation of mold?
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- 3) In some experiments, Serratia and the Bacillus are exposed to UV radiation for different duration to achieve sterilization. Why?1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection?1. Why do you disinfect your work area at the beginning of lab and then again at the end of lab? 2. If you have used proper aseptic technique to inoculate a new test tube of nutrient broth from apure culture of Bacillussubtilis, how many types of bacteria do you expect to grow in the new broth?And why?
- 15. Colony formation can be observed in liquid media. a) True b) False1. . Does a positive presumptive test mean that the water is absolutely unsafe to drink? Explain. 2. Describe the appearance of coliforms on Levine EMB agar. 3.. List five characteristics of coliform bacteria.1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, you are observing the growth of the plate prepared from the 10-8 plate. You divide the plate into two equal parts and count 46 colonies in each half.a) What was the dilution factor?b) How many total colonies were on the plate?c) Was your count valid?d) How many bacteria were present in the soil sample?
- 1. how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:1. What is the most common source of E. coli? (i.e. where does it occur naturally) 2. Why do children seem to be the most ill? (Give at least 2 reasons) 3. What is the morphology, morphological arrangement and Gram reaction of E. coli? 4. How does the E. coli strain 026:H11 damage its victims?1. Why should we not use toothpicks in streaking bacterial colonies in an agar plate? what should be used instead of toothpicks? 2. Enumerate at least two normal flora each living in the skin and in the intestines I will upvoteee
- 4. Which media can be used to isolate E. coli samples from contaminated lettuce? 5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea? 6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain. 7. What is the color or TSI for Salmonella? 8. What is a fastidious organism?6. Account for the fact that the C. sporogenes culture showed a separation within the medium or an elevation of the medium from the bottom of the test tube.1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…