1. What is the osmolality of animal cell culture medium with 0.3 M NaCl?

Three proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column equilibrated in a buffer with low ionic strength and eluted with an increasing gradient of salt in a buffer. Which protein will elute last from the column? 1. Protein with no charge. 2. Protein with eight negative charges. 3. Protein with five negative charges. 4. Both charged proteins will elute at the same time.

D10 Discuss some potential consequences for a patient using an unstable emulsion containing a hydrophobic drug

11. On the basis of the indicator test for starch what must have happened to the iodine molecules that were originally only in the water in the large test tube?

9. What relationship exists between molecular weight and the rate of diffusion?

16. What evidence do you have that diffusion occurs more rapidly under warmer conditions?

Please answer 1 and 2

define : a. isotonic slution b. hypertonic solution c. hypotonic solution

When the potato cube is surrounded by a hypotonic solution what change in mass occurs in the potato cells?

2. What will be the range of osmolality for most animal cell line culturing?